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Biotin Conjugated Mouse M-CSF/CSF1 Recombinant Rabbit Monoclonal Antibody [PSH15-85] - Detector

Usd: 409

Specification

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Catalog# HA723794B

Biotin Conjugated Mouse M-CSF/CSF1 Recombinant Rabbit Monoclonal Antibody [PSH15-85] - Detector

Application

  • ELISA(Det)

  • ELISA

Reactivity

  • Mouse

Pair: Capture
Pair: Detector
Pair: Protein
Range

  • 7.8-2,000 pg/mL

PI

  • 8.2

Conjugation

  • Biotin

Overview

Product Name

Biotin Conjugated Mouse M-CSF/CSF1 Recombinant Rabbit Monoclonal Antibody [PSH15-85] - Detector

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Mouse M-CSF/CSF1 aa 33-262 (HA211182).

Species Reactivity

Mouse

Validated Applications

ELISA(Det), ELISA

Positive Control

Recombinant Mouse M-CSF/CSF1 protein (HA211182).

Conjugation

Biotin

Clone Number

PSH15-85

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA(Det)

  • Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-84] to Mouse M-CSF/CSF1 antibody (Capture) (HA723792) and Recombinant Mouse M-CSF/CSF1 protein (HA211182) as the standard. The reference range value is 7.8-2,000 pg/mL.

  • ELISA

  • Use at an assay dependent concentration.

Target

Function

The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which causes hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. It may also be involved in development of the placenta. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.

Background References

1. Efe G et al. p53 Gain-of-Function Mutation Induces Metastasis via BRD4-Dependent CSF-1 Expression. Cancer Discov. 2023 Dec

2. Chitu V et al. Modeling CSF-1 receptor deficiency diseases - how close are we? FEBS J. 2022 Sep

Subcellular Location

Cell membrane; Secreted, extracellular space.

UNIPROT

SwissProt: P07141 Mouse

Synonyms

Colony stimulating factor 1 (macrophage) antibody

Colony stimulating factor 1 antibody

Colony stimulating factor macrophage specific antibody

CSF 1 antibody

CSF-1 antibody

CSF1 antibody

CSF1_HUMAN antibody

Csfm antibody

Lanimostim antibody

M CSF antibody

Expand

Images

  • Sandwich ELISA analysis of Mouse M-CSF/CSF1 matched pair antibodies<br /><br />Capture: <a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]<br />Detector: <a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse M-CSF/CSF1 protein (<a href="/products/HA211182" style="font-weight: bold;text-decoration: underline;">HA211182</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of Mouse M-CSF/CSF1 matched pair antibodies

    Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]
    Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]

    Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723792) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse M-CSF/CSF1 protein (HA211182) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723793, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated RAW264.7 cell culture supernatant.<br /><br />Capture: <a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]<br />Detector: <a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]<br /><br />RAW264.7 cells were stimulated with 1 µg/ml LPS and 30 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 24 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 731.4 pg/ml in LPS and recombinant mouse ING-γ stimulated RAW264.7 cell culture supernatant and undetectable in the unstimulated RAW264.7 control.

    Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated RAW264.7 cell culture supernatant.

    Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]
    Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]

    RAW264.7 cells were stimulated with 1 µg/ml LPS and 30 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 24 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 731.4 pg/ml in LPS and recombinant mouse ING-γ stimulated RAW264.7 cell culture supernatant and undetectable in the unstimulated RAW264.7 control.

  • Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated J774A.1 cell culture supernatant.<br /><br />Capture: <a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]<br />Detector: <a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]<br /><br />J774A.1 cells were stimulated with 1 µg/ml LPS and 100 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 72 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 704.2 pg/ml in LPS and recombinant mouse ING-γ stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.

    Interpolated concentrations of native M-CSF in mouse LPS and recombinant mouse ING-γ unstimulated and stimulated J774A.1 cell culture supernatant.

    Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]
    Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]

    J774A.1 cells were stimulated with 1 µg/ml LPS and 100 ng/ml recombinant mouse ING-γ or vehicle control and incubated for 72 hours. The concentrations of M-CSF measured in duplicate and interpolated from the M-CSF standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean M-CSF concentration was determined to be 704.2 pg/ml in LPS and recombinant mouse ING-γ stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.

  • Interpolated concentrations of spiked M-CSF in mouse cell culture media samples.<br /><br />Capture: <a href="/products/HA723792" style="font-weight: bold;text-decoration: underline;">HA723792</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]<br />Detector: <a href="/products/HA723793" style="font-weight: bold;text-decoration: underline;">HA723793</a>, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]<br /><br />The concentrations of M-CSF were measured in triplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).

    Interpolated concentrations of spiked M-CSF in mouse cell culture media samples.

    Capture: HA723792, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-84]
    Detector: HA723793, Mouse M-CSF/CSF1 Rabbit mAb [PSH15-85]

    The concentrations of M-CSF were measured in triplicates, interpolated from the M-CSF standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"