Biotin Conjugated Non-Phospho-Tau (T217) Recombinant Rabbit Monoclonal Antibody [PSH16-45] - Detector
Overview
Product Name
Biotin Conjugated Non-Phospho-Tau (T217) Recombinant Rabbit Monoclonal Antibody [PSH16-45] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Tau aa 5-24 (P10636-8).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Human Tau/Tau441, C-His (isoform 2N4R) Protein (HA210937).
Conjugation
Biotin
Clone Number
PSH16-45
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-44] to Human Non-Phospho-Tau (T217) antibody (Capture) (HA723844) and Recombinant Human Phospho-Tau (T217) protein (HA210937) as the standard. The reference range value is 0.117-30 ng/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity. Tau is highly subject to a variety of post-translational modifications, including phosphorylation on serine and threonine residues, polyubiquitination (and subsequent proteasomal degradation) and glycation of specific Tau isoforms. Defects in the gene encoding Tau are associated with Alzheimers disease, pallido-ponto-nigral degeneration (PPND), corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP).
Background References
1. Bayoumy S, Verberk IMW, den Dulk B, et al. Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231. Alzheimers Res Ther. 2021;13(1):198. Published 2021 Dec 4. doi:10.1186/s13195-021-00939-9
2. Janelidze S, Berron D, Smith R, et al. Associations of Plasma Phospho-Tau217 Levels With Tau Positron Emission Tomography in Early Alzheimer Disease. JAMA Neurol. 2021;78(2):149-156. doi:10.1001/jamaneurol.2020.4201
Subcellular Location
Cell membrane. Cytoplasm. Secreted.
UNIPROT
Synonyms
AI413597 antibody
AW045860 antibody
DDPAC antibody
FLJ31424 antibody
FTDP 17 antibody
G protein beta1/gamma2 subunit interacting factor 1 antibody
MAPT antibody
MAPTL antibody
MGC134287 antibody
MGC138549 antibody
ExpandImages
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![Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies<br /><br />Capture: <a href="/products/HA723844" style="font-weight: bold;text-decoration: underline;">HA723844</a>, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44]<br />Detector: <a href="/products/HA723209" style="font-weight: bold;text-decoration: underline;">HA723209</a>, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723844" style="font-weight: bold;text-decoration: underline;">HA723844</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (<a href="/products/HA210937" style="font-weight: bold;text-decoration: underline;">HA210937</a>) starting from 30000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723209" style="font-weight: bold;text-decoration: underline;">HA723209</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723846B_1.png?v=20260604174739)
Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies
Capture: HA723844, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44]
Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723844) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Non-p217 Tau in rat cerebellum, SH-SY5Y and MDA-MB-231 extract samples based on a 1000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA723844" style="font-weight: bold;text-decoration: underline;">HA723844</a>, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44]<br />Detector: <a href="/products/HA723209" style="font-weight: bold;text-decoration: underline;">HA723209</a>, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42]<br /><br />Interpolated concentration of native Non-p217 Tau was measured in duplicate at different sample concentrations and interpolated from the Non-p217 Tau standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Non-p217 Tau concentration was determined to be 35,404 pg/mL in SH-SY5Y cell extract, There was no detectable signal in MDA-MB-231 cell extract.](https://storage.huabio.cn/huabio/productImg/HA723846B_2.png?v=20260604174739)
Interpolated concentrations of native Non-p217 Tau in rat cerebellum, SH-SY5Y and MDA-MB-231 extract samples based on a 1000 µg/ml extract load.
Capture: HA723844, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44]
Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42]
Interpolated concentration of native Non-p217 Tau was measured in duplicate at different sample concentrations and interpolated from the Non-p217 Tau standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Non-p217 Tau concentration was determined to be 35,404 pg/mL in SH-SY5Y cell extract, There was no detectable signal in MDA-MB-231 cell extract. -
![Interpolated concentrations of spiked Non-p217 Tau in cell culture media samples.<br /><br />Capture: <a href="/products/HA723844" style="font-weight: bold;text-decoration: underline;">HA723844</a>, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44]<br />Detector: <a href="/products/HA723209" style="font-weight: bold;text-decoration: underline;">HA723209</a>, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42]<br /><br />The concentrations of Non-p217 Tau were measured in duplicates, interpolated from the Non-p217 Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723846B_3.png?v=20260604174739)
Interpolated concentrations of spiked Non-p217 Tau in cell culture media samples.
Capture: HA723844, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44]
Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42]
The concentrations of Non-p217 Tau were measured in duplicates, interpolated from the Non-p217 Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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