Mouse/Rat GDF15 Recombinant Rabbit Monoclonal Antibody [PSH16-65] - BSA and Azide free (Detector)

Sandwich ELISA analysis of Mouse GDF15 matched pair antibodies

Capture: HA723866, Mouse GDF15 Rabbit mAb [PSH16-64]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723866) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse GDF15 protein (HA211089) protein starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723867, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Mouse GDF15 matched pair antibodies

Capture: HA723868, Mouse GDF15 Rabbit mAb [PSH16-66]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723868) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse GDF15 protein (HA211089) protein starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA723867, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native GDF-15 in mouse and rat samples.

Capture: HA723866, Mouse GDF15 Rabbit mAb [PSH16-64]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

Interpolated concentration of native GDF-15 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 21,738 pg/mL in Hepa1-6 cell culture supernatant and 269 pg/mL in rat serum.

Interpolated concentrations of native GDF-15 in mouse and
rat samples.

Capture: HA723868, Mouse GDF15 Rabbit mAb [PSH16-66]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

Interpolated concentration of native GDF-15 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean GDF-15 concentration was determined to be 21,079 pg/mL in Hepa1-6 cell culture supernatant and 449 pg/mL in rat serum.

Interpolated concentrations of spiked GDF-15 in cell culture media samples.

Capture: HA723866, Mouse GDF15 Rabbit mAb [PSH16-64]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Interpolated concentrations of spiked GDF-15 in cell culture media samples.

Capture: HA723868, Mouse GDF15 Rabbit mAb [PSH16-66]
Detector: HA723867, Mouse GDF15 Rabbit mAb [PSH16-65]

The concentrations of GDF-15 were measured in duplicates, interpolated from the GDF-15 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).