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OTULIN Recombinant Rabbit Monoclonal Antibody [PSH16-95]

Usd: 385

Specification

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Catalog# HA723891

OTULIN Recombinant Rabbit Monoclonal Antibody [PSH16-95]

Application

  • WB

  • FC

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

OTULIN Recombinant Rabbit Monoclonal Antibody [PSH16-95]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human OTULIN aa 1-352.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 40 kDa

Positive Control

HeLa cell lysate, 786-0 cell lysate, U-2 OS.

Conjugation

unconjugated

Clone Number

PSH16-95

Reactivity Data

WB FC
Human
Tested Tested
Tested Tested
Mouse
Not Recommended
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • FC

  • 1:1,000

Target

Function

Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling.

Background References

1. Verboom L et al. OTULIN in NF-kappaB signaling, cell death, and disease. Trends Immunol. 2021 Jul

2. Dou B et al. OTULIN deficiency: focus on innate immune system impairment. Front Immunol. 2024 May

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q96BN8 Human

Synonyms

AIPDS antibody

Deubiquitinating enzyme otulin antibody

F105B_HUMAN antibody

Fam105b antibody

Family with sequence similarity 105, member B antibody

FLJ34884 antibody

GUM antibody

OTU deubiquitinase with linear linkage specificity antibody

OTULIN antibody

Protein FAM105B antibody

Expand

Images

  • Western blot analysis of OTULIN on different lysates with Rabbit anti-OTULIN antibody (<a href="/products/HA723891" style="font-weight: bold;text-decoration: underline;">HA723891</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 786-0 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 40 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723891" style="font-weight: bold;text-decoration: underline;">HA723891</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of OTULIN on different lysates with Rabbit anti-OTULIN antibody (HA723891) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 786-0 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 40 kDa
    Observed band size: 42 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723891) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of U-2 OS cells labeling OTULIN.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA723891" style="font-weight: bold;text-decoration: underline;">HA723891</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of U-2 OS cells labeling OTULIN.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA723891, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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