OTULIN Recombinant Rabbit Monoclonal Antibody [PSH16-95] - BSA and Azide free
Catalog# HA751609
OTULIN Recombinant Rabbit Monoclonal Antibody [PSH16-95] - BSA and Azide free
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WB
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FC
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Human
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HA723891
含抗保成分
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unconjugated
Overview
Product Name
OTULIN Recombinant Rabbit Monoclonal Antibody [PSH16-95] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human OTULIN aa 1-352.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 40 kDa
Positive Control
HeLa cell lysate, 786-0 cell lysate, U-2 OS.
Conjugation
unconjugated
Clone Number
PSH16-95
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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FC
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1:1,000
Target
Function
Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling.
Background References
1. Verboom L et al. OTULIN in NF-kappaB signaling, cell death, and disease. Trends Immunol. 2021 Jul
2. Dou B et al. OTULIN deficiency: focus on innate immune system impairment. Front Immunol. 2024 May
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
AIPDS antibody
Deubiquitinating enzyme otulin antibody
F105B_HUMAN antibody
Fam105b antibody
Family with sequence similarity 105, member B antibody
FLJ34884 antibody
GUM antibody
OTU deubiquitinase with linear linkage specificity antibody
OTULIN antibody
Protein FAM105B antibody
ExpandImages
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Western blot analysis of OTULIN on different lysates with Rabbit anti-OTULIN antibody (HA751609) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 786-0 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 42 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751609) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of U-2 OS cells labeling OTULIN.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751609, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"