ATF3 Recombinant Rabbit Monoclonal Antibody [PSH17-99]
Catalog# HA723964
ATF3 Recombinant Rabbit Monoclonal Antibody [PSH17-99]
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WB
-
Human
-
Mouse
-
HA751648
不含抗保成分
-
unconjugated
Overview
Product Name
ATF3 Recombinant Rabbit Monoclonal Antibody [PSH17-99]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ATF3 aa 1-100.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 21 kDa
Positive Control
HEK-293 cell lysate, THP-1 treated with 80nM TPA for 24 hours then treated with 1μg/mL LPS for 15 minutes cell lysate, RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH17-99
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
Cyclic AMP-dependent transcription factor ATF-3 is a protein that, in humans, is encoded by the ATF3 gene. ATF-3 is induced upon physiological stress in various tissues. It is also a marker of regeneration following injury of dorsal root ganglion neurons, as injured regenerating neurons activate this transcription factor. Functional validation studies have shown that ATF3 can promote regeneration of peripheral neurons, but is not capable of promoting regeneration of central nervous system neurons.
Background References
1. Hu S et al. Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis. Sci Adv. 2024 Jul
2. Huo S et al. ATF3/SPI1/SLC31A1 Signaling Promotes Cuproptosis Induced by Advanced Glycosylation End Products in Diabetic Myocardial Injury. Int J Mol Sci. 2023 Jan
Subcellular Location
Nucleus.
Synonyms
Activating transcription factor 3 antibody
ATF3 antibody
ATF3_HUMAN antibody
ATF3deltaZip2 antibody
ATF3deltaZip2c antibody
ATF3deltaZip3 antibody
cAMP dependent transcription factor ATF3 antibody
cAMP-dependent transcription factor ATF-3 antibody
Cyclic AMP dependent transcription factor ATF3 antibody
Cyclic AMP-dependent transcription factor ATF-3 antibody
Images
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☑ Relative expression (RE),Cell treatment (CT)
Western blot analysis of ATF3 on different lysates with Rabbit anti-ATF3 antibody (HA723964) at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: THP-1 cell lysate
Lane 4: THP-1 treated with 80nM TPA for 24 hours then treated with 1μg/mL LPS for 15 minutes cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate
Lane 7: NIH/3T3 cell lysate (negative)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21/19 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723964) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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