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ATF3 Recombinant Rabbit Monoclonal Antibody [PSH17-99] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751648

ATF3 Recombinant Rabbit Monoclonal Antibody [PSH17-99] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ATF3 Recombinant Rabbit Monoclonal Antibody [PSH17-99] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ATF3 aa 1-100.

Species Reactivity

Human, Mouse

Validated Applications

WB

Target Molecular Weight

Predicted band size: 21 kDa

Positive Control

HEK-293 cell lysate, THP-1 treated with 80nM TPA for 24 hours then treated with 1μg/mL LPS for 15 minutes cell lysate, RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH17-99

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

Cyclic AMP-dependent transcription factor ATF-3 is a protein that, in humans, is encoded by the ATF3 gene. ATF-3 is induced upon physiological stress in various tissues. It is also a marker of regeneration following injury of dorsal root ganglion neurons, as injured regenerating neurons activate this transcription factor. Functional validation studies have shown that ATF3 can promote regeneration of peripheral neurons, but is not capable of promoting regeneration of central nervous system neurons.

Background References

1. Hu S et al. Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis. Sci Adv. 2024 Jul

2. Huo S et al. ATF3/SPI1/SLC31A1 Signaling Promotes Cuproptosis Induced by Advanced Glycosylation End Products in Diabetic Myocardial Injury. Int J Mol Sci. 2023 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P18847 Human

SwissProt: Q60765 Mouse

Synonyms

Activating transcription factor 3 antibody

ATF3 antibody

ATF3_HUMAN antibody

ATF3deltaZip2 antibody

ATF3deltaZip2c antibody

ATF3deltaZip3 antibody

cAMP dependent transcription factor ATF3 antibody

cAMP-dependent transcription factor ATF-3 antibody

Cyclic AMP dependent transcription factor ATF3 antibody

Cyclic AMP-dependent transcription factor ATF-3 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE),Cell treatment (CT)</span><br /><br />Western blot analysis of ATF3 on different lysates with Rabbit anti-ATF3 antibody (<a href="/products/HA751648" style="font-weight: bold;text-decoration: underline;">HA751648</a>) at 1/5,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: MCF7 cell lysate (negative)<br />Lane 3: THP-1 cell lysate<br />Lane 4: THP-1 treated with 80nM TPA for 24 hours then treated with 1μg/mL LPS for 15 minutes cell lysate<br />Lane 5: RAW264.7 cell lysate<br />Lane 6: RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate<br />Lane 7: NIH/3T3 cell lysate (negative)<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21/19 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751648" style="font-weight: bold;text-decoration: underline;">HA751648</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE),Cell treatment (CT)

    Western blot analysis of ATF3 on different lysates with Rabbit anti-ATF3 antibody (HA751648) at 1/5,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: MCF7 cell lysate (negative)
    Lane 3: THP-1 cell lysate
    Lane 4: THP-1 treated with 80nM TPA for 24 hours then treated with 1μg/mL LPS for 15 minutes cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: RAW264.7 treated with 1μg/mL LPS for 24 hours cell lysate
    Lane 7: NIH/3T3 cell lysate (negative)

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 21/19 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751648) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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ATF3 Rabbit Polyclonal Antibody

Application: WB,IHC-P,IF-Tissue

Reactivity: Human,Mouse

Conjugate: unconjugated

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ATF3 Recombinant Rabbit Monoclonal Antibody [PSH17-99]

Application: WB

Reactivity: Human,Mouse

Conjugate: unconjugated