MURF1 Recombinant Rabbit Monoclonal Antibody [PSH18-10]
Catalog# HA723973
MURF1 Recombinant Rabbit Monoclonal Antibody [PSH18-10]
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WB
-
FC
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MURF1 Recombinant Rabbit Monoclonal Antibody [PSH18-10]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MURF1 aa 180-343 /353
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC, IP
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
SK-MEL-28 cell lysate, Mouse skeletal muscle tissue lysate, Mouse heart tissue lysate, Rat skeletal muscle tissue lysate, Rat heart tissue lysate, SK-MEL-28.
Conjugation
unconjugated
Clone Number
PSH18-10
Reactivity Data
| WB | FC | IP | |
|---|---|---|---|
| Human |
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| Mouse |
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|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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FC
-
1:1,000
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IP
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1-2μg/sample
Target
Function
E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Background References
1. Dai KS et al. A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain. J Biol Chem. 2001
2. McElhinny AS et al. Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1. J Cell Biol. 2002
Subcellular Location
Cytoplasm, Nucleus, myofibril, sarcomere, M line, Z line.
Synonyms
IRF antibody
MURF1 antibody
RNF28 antibody
SMRZ antibody
TRIM63 antibody
E3 ubiquitin-protein ligase TRIM63 antibody
Iris RING finger protein antibody
Muscle-specific RING finger protein 1 antibody
RING finger protein 28 antibody
RING-type E3 ubiquitin transferase TRIM63 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of MURF1 on different lysates with Rabbit anti-MURF1 antibody (HA723973) at 1/5,000 dilution.
Lane 1: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 2: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 3: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 4: Mouse heart tissue lysate (40 µg/Lane)
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time:1 minute 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723973) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Western blot analysis of MURF1 on different lysates with Rabbit anti-MURF1 antibody (HA723973) at 1/5,000 dilution.
Lane 1: Rat skeletal muscle tissue lysate (40 µg/Lane)
Lane 2: Rat liver tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723973) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of SK-MEL-28 cells labeling MURF1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723973, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
MURF1 was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with HA723973 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723973 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-MEL-28 cell lysate (input)
Lane 2: HA723973 IP in SK-MEL-28 cell lysate
Lane 3: Rabbit IgG instead of HA723973 in SK-MEL-28 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 10 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
MURF1 Recombinant Rabbit Monoclonal Antibody [PSH18-10] - BSA and Azide free
Application: WB,FC,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
