Biotin Conjugated Human/Monkey ACE Recombinant Rabbit Monoclonal Antibody [PSH22-82] - Detector

Sandwich ELISA analysis of Human ACE matched pair antibodies

Capture: HA724326, Human/Monkey ACE Rabbit mAb [PSH22-81]
Detector: HA724327, Human/Monkey ACE Rabbit mAb [PSH22-82]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724326) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ACE protein (HA211209) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA724327, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ACE in Human serum.

Capture: HA724326, Human/Monkey ACE Rabbit mAb [PSH22-81]
Detector: HA724327, Human/Monkey ACE Rabbit mAb [PSH22-82]

Interpolated concentration of native ACE was measured in duplicate at different sample concentrations and interpolated from the ACE standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ACE concentration was determined to be 224,145 pg/ml in human serum.

Interpolated concentrations of native ACE in Monkey serum.

Capture: HA724326, Human/Monkey ACE Rabbit mAb [PSH22-81]
Detector: HA724327, Human/Monkey ACE Rabbit mAb [PSH22-82]

Interpolated concentration of native ACE was measured in duplicate at different sample concentrations and interpolated from the ACE standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ACE concentration was determined to be 100,598 pg/ml in monkey serum.

Interpolated concentrations of spiked ACE in cell culture media samples.

Capture: HA724326, Human/Monkey ACE Rabbit mAb [PSH22-81]
Detector: HA724327, Human/Monkey ACE Rabbit mAb [PSH22-82]

The concentrations of ACE were measured in duplicates, interpolated from the ACE standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).