Biotin Conjugated Human Ki67 Recombinant Rabbit Monoclonal Antibody [SR00-02] - Detector
Overview
Product Name
Biotin Conjugated Human Ki67 Recombinant Rabbit Monoclonal Antibody [SR00-02] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Ki67 aa 1,000-1,234 (HA211578).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Ki67 protein (HA211578).
Conjugation
Biotin
Clone Number
SR00-02
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH25-46] to Human Ki67 (Capture) (HA724536) and Recombinant Human Ki67 protein (HA211578) as the standard. The reference range value is 31.3-8,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
The Ki-67 protein is a nuclear protein doublet, 345-395 kDa, playing a pivotal role in maintaining cell proliferation. Ki-67 is present in all non-G0 phases of the cell cycle. Beginning in the mid G1, the level increases through S and G2 to reach a peak in M. In the end of M, is is rapidly catabolized. The Ki-67 labelling index (LI), i.e., the percentage of cells in a tissue staining for Ki-67, indicates the growth fraction. For many tumours, the rate of cell proliferation as assessed by Ki-67 immunoreactivity correlates with tumour grade and clinical course. In Non-Hodgkin lymphoma a labelling index of less than 20% is seen in low grade lymphomas, greater than 20% is associated with high grade lymphomas. Low grade lymphomas with a labelling index in excess of 5% have a worse prognosis than those with an index of less than 5%. In Burkitt and Burkitt-like lymphoma, nearly 100% of the nuclei are stained. This can be used as a diagnostic criterion. In gliomas the indices ranges from 0% to 5% for low grade astrocytomas while anaplastic astrocytomas and glioblastomas most frequently show an index above 10%. In soft tissue sarcomas Ki-67 index is positively correlated with mitotic count, cellularity and histological grade. In some benign tumours, like meningioma, a high LI is associated with a high recurrence rate. In dysplasia in Barrett's oesophagus and in granulosa cell tumours and ovarian serous tumours, Ki-67 LI is associated with progression. In the former, reproducibility of dysplasia grading is improved when Ki67 is included. In breast cancer, the proliferative index measured by Ki67 immunoreactivity has both prognostic and predictive value.
Background References
1. Cuylen S. et al. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes. Nature 535:308-312(2016).
2. Booth D.G. et al. Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery. Elife 3:E01641-E01641(2014).
Subcellular Location
Chromosome, Nucleus, nucleolus.
UNIPROT
Synonyms
Antigen identified by monoclonal antibody Ki 67 antibody
Antigen identified by monoclonal antibody Ki-67 antibody
Antigen KI-67 antibody
Antigen KI67 antibody
Antigen Ki67 antibody
KI67_HUMAN antibody
KIA antibody
Marker of proliferation Ki-67 antibody
MIB 1 antibody
MIB antibody
ExpandImages
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![Sandwich ELISA analysis of Human Ki67 matched pair antibodies<br /><br />Capture: <a href="/products/HA724536" style="font-weight: bold;text-decoration: underline;">HA724536</a>, Human Ki67 Rabbit mAb [PSH25-46]<br />Detector: <a href="/products/HA724537" style="font-weight: bold;text-decoration: underline;">HA724537</a>, Human Ki67 Rabbit mAb [SR00-02]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA724536" style="font-weight: bold;text-decoration: underline;">HA724536</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Ki67 protein (HA211578) starting from 8,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA724537" style="font-weight: bold;text-decoration: underline;">HA724537</a>, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA724537B_1.png?v=20260618231232)
Sandwich ELISA analysis of Human Ki67 matched pair antibodies
Capture: HA724536, Human Ki67 Rabbit mAb [PSH25-46]
Detector: HA724537, Human Ki67 Rabbit mAb [SR00-02]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724536) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Ki67 protein (HA211578) starting from 8,000 pg/mL to 0 pg/mL and detect antibody (HA724537, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Ki67 in A431 and HeLa cell extract samples based on a 1,000 µg/mL extract load.<br /><br />Capture: <a href="/products/HA724536" style="font-weight: bold;text-decoration: underline;">HA724536</a>, Human Ki67 Rabbit mAb [PSH25-46]<br />Detector: <a href="/products/HA724537" style="font-weight: bold;text-decoration: underline;">HA724537</a>, Human Ki67 Rabbit mAb [SR00-02]<br /><br />The concentrations of Ki67 were measured in duplicates, interpolated from the Ki67 standard curve and corrected for sample dilution. Undiluted samples are A431 cell extract 50% and HeLa cell extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ki67 concentration was determined to be 13,077 pg/mL in A431 cell extract and 39,701 pg/mL in HeLa cell extract.](https://storage.huabio.cn/huabio/productImg/HA724537B_2.png?v=20260618231232)
Interpolated concentrations of native Ki67 in A431 and HeLa cell extract samples based on a 1,000 µg/mL extract load.
Capture: HA724536, Human Ki67 Rabbit mAb [PSH25-46]
Detector: HA724537, Human Ki67 Rabbit mAb [SR00-02]
The concentrations of Ki67 were measured in duplicates, interpolated from the Ki67 standard curve and corrected for sample dilution. Undiluted samples are A431 cell extract 50% and HeLa cell extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ki67 concentration was determined to be 13,077 pg/mL in A431 cell extract and 39,701 pg/mL in HeLa cell extract. -
![Interpolated concentrations of spiked Ki67 in human cell culture media samples.<br /><br />Capture: <a href="/products/HA724536" style="font-weight: bold;text-decoration: underline;">HA724536</a>, Human Ki67 Rabbit mAb [PSH25-46]<br />Detector: <a href="/products/HA724537" style="font-weight: bold;text-decoration: underline;">HA724537</a>, Human Ki67 Rabbit mAb [SR00-02]<br /><br />The concentrations of Ki67 were measured in duplicates, interpolated from the Ki67 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA724537B_3.png?v=20260618231232)
Interpolated concentrations of spiked Ki67 in human cell culture media samples.
Capture: HA724536, Human Ki67 Rabbit mAb [PSH25-46]
Detector: HA724537, Human Ki67 Rabbit mAb [SR00-02]
The concentrations of Ki67 were measured in duplicates, interpolated from the Ki67 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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