Skip to content

CD10 Recombinant Rabbit Monoclonal Antibody [PSH28-45]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA724782

CD10 Recombinant Rabbit Monoclonal Antibody [PSH28-45]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CD10 Recombinant Rabbit Monoclonal Antibody [PSH28-45]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 86 kDa

Positive Control

Raji (Human Burkitt's lymphoma cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate.

Conjugation

unconjugated

Clone Number

PSH28-45

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC
human
Tested Tested
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

0.5 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:500

Target

Function

CD10, also called the common acute lymphoblastic leukemia antigen (CALLA) and neutral endopeptidase (NEP), is a type II integral membrane glycoprotein. CD10 acts as a zinc metalloprotease that cleaves a variety of biologically active peptides including Angiotensins I and II. It is expressed on early B and T lymphoid precursors, B blasts, some granulocytes, bone marrow stromal cells and certain epithelial cells including some tumor cell lines. CD10 is used as a marker of common acute lymphocytic leukemias and some lymphomas.

Background References

1. Kim, TH. et al. 2015. Expression of p27 and Jun activation domain-binding protein 1 in endometriosis. Arch. Gynecol. Obstet. 292: 377-81.

2. Engel, BJ. et al. 2015. Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures. Adv Healthc Mater. 4: 1664-74.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P08473 Human

Synonyms

Atriopeptidase antibody

CALLA antibody

CD10 antibody

CD10 antigen antibody

Common acute lymphocytic leukemia antigen antibody

DKFZp686O16152 antibody

EC 3.4.24.11 antibody

Enkephalinase antibody

EPN antibody

Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (<a href="/products/HA724782" style="font-weight: bold;text-decoration: underline;">HA724782</a>) at 1/2,500 dilution. <br /> <br />Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate <br />Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate <br />Lane 3: LNCaP (Human prostate cancer cell) cell lysate <br />Lane 4: Ramos (Human Burkitt's lymphoma cell) cell lysate <br /> <br />Lysates/proteins at 10 µg/Lane. <br />Exposure time: 20 seconds; ECL: K1801 <br /> <br />Jurkat is a negative control (PMID: 1396581). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724782" style="font-weight: bold;text-decoration: underline;">HA724782</a>, 1/2,500 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 86 kDa <br />Observed band size: 100 kDa

    ☑ Relative expression (RE)

    Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (HA724782) at 1/2,500 dilution.

    Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate
    Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 3: LNCaP (Human prostate cancer cell) cell lysate
    Lane 4: Ramos (Human Burkitt's lymphoma cell) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Jurkat is a negative control (PMID: 1396581).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724782, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 86 kDa
    Observed band size: 100 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724782" style="font-weight: bold;text-decoration: underline;">HA724782</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724782, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Prostate <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724782" style="font-weight: bold;text-decoration: underline;">HA724782</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Prostate
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724782, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724782" style="font-weight: bold;text-decoration: underline;">HA724782</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724782, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Flow Cytometry <br /> <br />Species: Human <br />Sample: LNCaP (Human prostate cancer cell) <br /> <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA724782" style="font-weight: bold;text-decoration: underline;">HA724782</a> (1 μg/mL, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.

    Application: Flow Cytometry

    Species: Human
    Sample: LNCaP (Human prostate cancer cell)

    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724782 (1 μg/mL, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

CD10 Mouse Monoclonal Antibody [A1G3]

Application: WB,IHC-P,FC,IF-Tissue

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

CD10 Recombinant Rabbit Monoclonal Antibody [SN75-07] - BSA and Azide free

Application: WB,FC,IHC-P,IF-Cell,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

CD10 Rabbit Polyclonal Antibody

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

CD10 Mouse Monoclonal Antibody

Application: mIHC

Reactivity: Human

Conjugate: unconjugated

metafield image

CD10 Recombinant Rabbit Monoclonal Antibody [SN75-07]

Application: WB,FC,IHC-P,IF-Cell,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

CD10 Mouse Monoclonal Antibody [A1G5]

Application: WB,IHC-P,IF-Tissue,FC,mIHC

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

CD10 Recombinant Rabbit Monoclonal Antibody [JE60-45]

Application: WB,IHC-P

Reactivity: Human

Conjugate: unconjugated

metafield image

CD10 Recombinant Mouse Monoclonal Antibody [A1G3-R] - BSA and Azide free

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

CD10 Mouse Monoclonal Antibody [A1G4]

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

CD10 Recombinant Mouse Monoclonal Antibody [A1G3-R]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated