Biotin Conjugated Human Angiogenin Recombinant Rabbit Monoclonal Antibody [PSH12-65] - Detector
Overview
Product Name
Biotin Conjugated Human Angiogenin Recombinant Rabbit Monoclonal Antibody [PSH12-65] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Angiogenin aa 24-147 (HA210986).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Angiogenin protein (HA210986).
Conjugation
Biotin
Clone Number
PSH12-65
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-64] to Human Angiogenin antibody (Capture) (HA725076) and Recombinant Human Angiogenin protein (HA210986) as the standard. The reference range value is 23.4-3,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
The protein encoded by this gene is a member of the RNase A superfamily though it has relatively weak ribonucleolytic activity. This protein is a potent mediator of new blood vessel formation and thus, in addition to the name RNase5, is commonly called angiogenin. This protein induces angiogenesis after binding to actin on the surface of endothelial cells. This protein also accumulates at the nucleolus where it stimulates ribosomal transcription. Under stress conditions this protein translocates to the cytosol where it hydrolyzes cellular tRNAs and influences protein synthesis. A signal peptide is cleaved from the precursor protein to produce a mature protein which contains a nuclear localization signal, a cell binding motif, and a catalytic domain. This protein has been shown to be both neurotrophic and neuroprotective and the mature protein has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Due to its effect on rRNA production and angiogenesis this gene plays important roles in cell growth and tumor progression. Mutations in this gene are associated with progression of amyotrophic lateral sclerosis (ALS). This gene and the neighboring RNase4 gene share promoters and 5' exons though each gene then splices to a distinct 3' exon containing the complete coding region of each gene. Alternative splicing results in multiple transcript variants encoding the same protein.
Background References
1. Bai R., Sun D., Chen M., Shi X., Luo L., Yao Z., Liu Y., Ge X., Gao X., Hu G.F., Zhou W., Sheng J., Xu Z. Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis. EMBO J. 39:e103325-e103325 (2020)
2. Thomas S.P., Hoang T.T., Ressler V.T., Raines R.T. Human angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor. RNA 24:1018-1027 (2018)
Subcellular Location
Secreted.
UNIPROT
Synonyms
ALS9 antibody
AMYOTROPHIC LATERAL SCLEROSIS antibody
ANG 1 antibody
ANG antibody
ANG I antibody
ANG1 antibody
ANGI antibody
ANGI_HUMAN antibody
Angiogenin antibody
Angiogenin ribonuclease RNase A family, 5 antibody
ExpandImages
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![Sandwich ELISA analysis of human Angiogenin matched pair antibodies<br /><br />Capture: <a href="/products/HA725076" style="font-weight: bold;text-decoration: underline;">HA725076</a>, Human Angiogenin Rabbit mAb [PSH12-64]<br />Detector: <a href="/products/HA725077" style="font-weight: bold;text-decoration: underline;">HA725077</a>, Human Angiogenin Rabbit mAb [PSH12-65]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725076" style="font-weight: bold;text-decoration: underline;">HA725076</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein (<a href="/products/HA210986" style="font-weight: bold;text-decoration: underline;">HA210986</a>) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725077" style="font-weight: bold;text-decoration: underline;">HA725077</a>, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725078B_1.png?v=20260604174820)
Sandwich ELISA analysis of human Angiogenin matched pair antibodies
Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64]
Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725076) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein (HA210986) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA725077, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Angiogenin in HepG2, HT-29 and MCF7 cell culture supernatant.<br /><br />Capture: <a href="/products/HA725076" style="font-weight: bold;text-decoration: underline;">HA725076</a>, Human Angiogenin Rabbit mAb [PSH12-64]<br />Detector: <a href="/products/HA725077" style="font-weight: bold;text-decoration: underline;">HA725077</a>, Human Angiogenin Rabbit mAb [PSH12-65]<br /><br />The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 2.5%, HT-29 cell culture supernatant 100% and MCF7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 19,596 pg/ml in HepG2 cell culture supernatant, 151 pg/ml in HT-29 cell culture supernatant and undetectable in MCF7 cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA725078B_2.png?v=20260604174820)
Interpolated concentrations of native Angiogenin in HepG2, HT-29 and MCF7 cell culture supernatant.
Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64]
Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65]
The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 2.5%, HT-29 cell culture supernatant 100% and MCF7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 19,596 pg/ml in HepG2 cell culture supernatant, 151 pg/ml in HT-29 cell culture supernatant and undetectable in MCF7 cell culture supernatant.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human Angiogenin Recombinant Rabbit Monoclonal Antibody [PSH12-64] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
Human Angiogenin Recombinant Rabbit Monoclonal Antibody [PSH12-65] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
