Human CCL11/Eotaxin Recombinant Rabbit Monoclonal Antibody [PSH16-25] - BSA and Azide free (Detector)
Overview
Product Name
Human CCL11/Eotaxin Recombinant Rabbit Monoclonal Antibody [PSH16-25] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CCL11/Eotaxin aa 24-97 (HA210865).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human CCL11/Eotaxin protein (HA210865).
Conjugation
unconjugated
Clone Number
PSH16-25
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH16-24] to Human CCL11/Eotaxin antibody (Capture) (HA725197) and Recombinant Human CCL11/Eotaxin protein (HA210865) as the standard. The reference range value is 15.6-4,000 pg/mL.
Target
Function
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.
Background References
1. Garcia-Zepeda E.A., Rothenberg M.E., Ownbey T.R., Leder P., Luster A.D. Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nat. Med. 2:449-456 (1996)
2. Kitaura M., Nakajima T., Imai T., Harada S., Combadiere C., Tiffany H.L., Murphy P.M., Yoshie O. Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J. Biol. Chem. 271:7725-7730 (1996)
Subcellular Location
Secreted.
UNIPROT
Synonyms
C-C motif chemokine 11 antibody
CCL 11 antibody
CCL11 antibody
CCL11_HUMAN antibody
chemokine (C-C motif) ligand 11 antibody
Chemokine C C motif ligand 11 antibody
Chemokine CC Motif Ligand 11 antibody
Chemokine ligand 11 antibody
Eosinophil chemotactic protein antibody
Eotaxin antibody
ExpandImages
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![Sandwich ELISA analysis of Human CCL11/Eotaxin matched pair antibodies<br /><br />Capture: <a href="/products/HA725197" style="font-weight: bold;text-decoration: underline;">HA725197</a>, Human CCL11/Eotaxin Rabbit mAb [PSH16-24]<br />Detector: <a href="/products/HA725198" style="font-weight: bold;text-decoration: underline;">HA725198</a>, Human CCL11/Eotaxin Rabbit mAb [PSH16-25]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725197" style="font-weight: bold;text-decoration: underline;">HA725197</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL11/Eotaxin protein (<a href="/products/HA210865" style="font-weight: bold;text-decoration: underline;">HA210865</a>) protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725198" style="font-weight: bold;text-decoration: underline;">HA725198</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725198_1.png?v=20260401003140)
Sandwich ELISA analysis of Human CCL11/Eotaxin matched pair antibodies
Capture: HA725197, Human CCL11/Eotaxin Rabbit mAb [PSH16-24]
Detector: HA725198, Human CCL11/Eotaxin Rabbit mAb [PSH16-25]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725197) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL11/Eotaxin protein (HA210865) protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725198, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CCL11 in human serum samples.<br /><br />Capture: <a href="/products/HA725197" style="font-weight: bold;text-decoration: underline;">HA725197</a>, Human CCL11/Eotaxin Rabbit mAb [PSH16-24]<br />Detector: <a href="/products/HA725198" style="font-weight: bold;text-decoration: underline;">HA725198</a>, Human CCL11/Eotaxin Rabbit mAb [PSH16-25]<br /><br />The concentrations of CCL11 were measured in duplicates, interpolated from the CCL11 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 122.41 pg/ml in human serum.](https://storage.huabio.cn/huabio/productImg/HA725198_2.png?v=20260401003140)
Interpolated concentrations of native CCL11 in human serum samples.
Capture: HA725197, Human CCL11/Eotaxin Rabbit mAb [PSH16-24]
Detector: HA725198, Human CCL11/Eotaxin Rabbit mAb [PSH16-25]
The concentrations of CCL11 were measured in duplicates, interpolated from the CCL11 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean serum concentration was determined to be 122.41 pg/ml in human serum.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human CCL11/Eotaxin Recombinant Rabbit Monoclonal Antibody [PSH16-24] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Cynomolgus monkey
Conjugate: unconjugated
