Human/Monkey CCL11/Eotaxin enzyme-linked immunoassay kit
Catalog# EH0201
Human/Monkey CCL11/Eotaxin enzyme-linked immunoassay kit
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ELISA
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Human
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62.5~4000 pg/ml
Overview
Product Name
Human/Monkey CCL11/Eotaxin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
4.73 pg/ml (50 μl)；8.97 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0179 | 0.0182 | 0.0181 | |
| 62.50 | 0.0510 | 0.0524 | 0.0517 | 0.0337 |
| 125.00 | 0.0924 | 0.0940 | 0.0932 | 0.0752 |
| 250.00 | 0.1999 | 0.2008 | 0.2004 | 0.1823 |
| 500.00 | 0.4787 | 0.4712 | 0.4750 | 0.4569 |
| 1000.00 | 1.1480 | 1.1570 | 1.1525 | 1.1345 |
| 2000.00 | 2.7190 | 2.5540 | 2.6365 | 2.6185 |
| 4000.00 | 4.4816 | 4.4996 | 4.4906 | 4.4726 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 111.0 | 495.8 | 1676.5 | 100.0 | 402.4 | 1352.0 |
| Standard Deviation | 6.2 | 23.4 | 93.6 | 4.1 | 19.1 | 74.6 |
| Coefficient of Variation (%) | 5.6 | 4.7 | 5.6 | 4.1 | 4.7 | 5.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CCL11/Eotaxin，未加人 CCL11/Eotaxin 的血清作为本底，计算回收率。回收率的范围从 104 % 至 120 %，平均回收率为 117%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-151.13 | 57 | 38.4 |
应用本试剂盒，检测30份健康人和2份健康猴的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C-C motif chemokine 11
CCL 11
CCL11
CCL11_HUMAN
chemokine (C-C motif) ligand 11
Chemokine C C motif ligand 11
Chemokine CC Motif Ligand 11
Chemokine ligand 11
Eosinophil chemotactic protein
Eotaxin
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