Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)
Catalog# EHY0202
Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.78~50 μg/ml
Overview
Product Name
Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.09 pg/ml (10 μl)
| μg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0079 | 0.0072 | 0.0076 | |
| 0.78 | 0.0272 | 0.0261 | 0.0267 | 0.0191 |
| 1.56 | 0.0599 | 0.0576 | 0.0588 | 0.0512 |
| 3.13 | 0.1500 | 0.1343 | 0.1422 | 0.1346 |
| 6.25 | 0.3914 | 0.3644 | 0.3779 | 0.3704 |
| 12.50 | 0.9581 | 0.9236 | 0.9409 | 0.9333 |
| 25.00 | 2.0780 | 2.1880 | 2.1330 | 2.1255 |
| 50.00 | 3.1490 | 3.1720 | 3.1605 | 3.1530 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (μg/ml) | 0.4 | 2.0 | 6.2 | 0.4 | 2.0 | 6.7 |
| Standard Deviation | 0.0 | 0.1 | 0.3 | 0.0 | 0.1 | 0.2 |
| Coefficient of Variation (%) | 4.9 | 4.1 | 4.6 | 4.0 | 3.4 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human/monkey Complement C3 into health human/monkey serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 99% to 120% with an overall mean recovery of 113%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (mg/ml) | Detectable (%) | Mean of Detectable (mg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 0.89-6.30 | 100 | 2.18 |
| Monkey Serum | 2 | 1.89-2.06 | 100 | 1.98 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and two samples from healthy monkeys were evaluated for the presence of human/monkey Complement C3 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Acylation stimulating protein cleavage product
AHUS5
ARMD9
ASP
C3 and PZP like alpha 2 macroglobulin domain containing protein 1
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
C3
CO3_HUMAN
Complement C3
Complement C3c alpha' chain fragment 2
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