Human/Monkey YKL-40/CHI3L1 enzyme-linked immunoassay kit(one step)
Catalog# EHY0159
Human/Monkey YKL-40/CHI3L1 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.14~100 ng/ml
Overview
Product Name
Human/Monkey YKL-40/CHI3L1 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.01 ng/ml (50 μl)；0.05 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0064 | 0.0057 | 0.0061 | |
| 0.14 | 0.0142 | 0.0134 | 0.0138 | 0.0078 |
| 0.41 | 0.0331 | 0.0298 | 0.0315 | 0.0254 |
| 1.23 | 0.0889 | 0.0861 | 0.0875 | 0.0815 |
| 3.70 | 0.2568 | 0.2291 | 0.2430 | 0.2369 |
| 11.11 | 0.7598 | 0.7190 | 0.7394 | 0.7334 |
| 33.33 | 1.8610 | 1.8180 | 1.8395 | 1.8335 |
| 100.00 | 3.3150 | 3.3390 | 3.3270 | 3.3210 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.8 | 9.7 | 32.4 | 1.8 | 9.2 | 31.1 |
| Standard Deviation | 0.0 | 0.3 | 1.4 | 0.1 | 0.4 | 1.8 |
| Coefficient of Variation (%) | 2.6 | 3.1 | 4.4 | 4.2 | 4.3 | 5.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human YKL-40/CHI3L1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 118% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 21.98-59.03 | 100.0 | 35.37 |
| Monkey Serum | 2 | 125.95-140.71 | 100 | 133.33 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and two samples from healthy monkeys were evaluated for the presence of human YKL-40/CHI3L1 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
39 kDa synovial protein
ASRT7
Cartilage glycoprotein 39
CGP-39
CGP39
CH3L1_HUMAN
CHI3L1
chitinase 3 like 1 (cartilage glycoprotein 39)
chitinase 3 like 1
Chitinase 3 like protein 1 precursor
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