Human/Monkey YKL-40/CHI3L1 enzyme-linked immunoassay kit(one step)
Catalog# EHY0159
Human/Monkey YKL-40/CHI3L1 enzyme-linked immunoassay kit(one step)
-
ELISA
-
Human
-
0.14~100 ng/ml
Overview
Product Name
Human/Monkey YKL-40/CHI3L1 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.01 ng/ml (50 μl)；0.05 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0064 | 0.0057 | 0.0061 | |
| 0.14 | 0.0142 | 0.0134 | 0.0138 | 0.0078 |
| 0.41 | 0.0331 | 0.0298 | 0.0315 | 0.0254 |
| 1.23 | 0.0889 | 0.0861 | 0.0875 | 0.0815 |
| 3.70 | 0.2568 | 0.2291 | 0.2430 | 0.2369 |
| 11.11 | 0.7598 | 0.7190 | 0.7394 | 0.7334 |
| 33.33 | 1.8610 | 1.8180 | 1.8395 | 1.8335 |
| 100.00 | 3.3150 | 3.3390 | 3.3270 | 3.3210 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.8 | 9.7 | 32.4 | 1.8 | 9.2 | 31.1 |
| Standard Deviation | 0.0 | 0.3 | 1.4 | 0.1 | 0.4 | 1.8 |
| Coefficient of Variation (%) | 2.6 | 3.1 | 4.4 | 4.2 | 4.3 | 5.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 YKL-40/CHI3L1，未加人 YKL-40/CHI3L1 的血清作为本底，计算回收率。回收率的范围从 87 % 至 118 %，平均回收率为 105%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 21.98-59.03 | 100 | 35.37 |
应用本试剂盒，检测30份健康人和2份健康猴的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
39 kDa synovial protein
ASRT7
Cartilage glycoprotein 39
CGP-39
CGP39
CH3L1_HUMAN
CHI3L1
chitinase 3 like 1 (cartilage glycoprotein 39)
chitinase 3 like 1
Chitinase 3 like protein 1 precursor
Expand