Human/Monkey TIMP4 enzyme-linked immunoassay kit
Catalog# EH0326
Human/Monkey TIMP4 enzyme-linked immunoassay kit
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ELISA
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Human
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Monkey
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78.13 ~5000 pg/ml
Overview
Product Name
Human/Monkey TIMP4 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
28.52 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0065 | 0.0059 | 0.0062 | |
| 78.13 | 0.0104 | 0.0093 | 0.0099 | 0.0037 |
| 156.25 | 0.0222 | 0.0235 | 0.0229 | 0.0167 |
| 312.50 | 0.0681 | 0.0700 | 0.0691 | 0.0629 |
| 625.00 | 0.2328 | 0.2283 | 0.2306 | 0.2244 |
| 1250.00 | 0.6881 | 0.6859 | 0.6870 | 0.6808 |
| 2500.00 | 1.8830 | 1.6560 | 1.7695 | 1.7633 |
| 5000.00 | 3.2420 | 3.1380 | 3.1900 | 3.1838 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 93.6 | 480.5 | 1404.5 | 116.4 | 528.0 | 1584.7 |
| Standard Deviation | 5.3 | 24.8 | 67.8 | 3.6 | 12.1 | 63.3 |
| Coefficient of Variation (%) | 5.6 | 5.2 | 4.8 | 3.1 | 2.3 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human/monkey TIMP4 into health human/monkey serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 119% with an overall mean recovery of 100%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 270.00-1131.02 | 100.0 | 740.27 |
| Monkey Serum | 1 | 895.85 | 100.0 | 895.85 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and one sample from healthy monkey were evaluated for the presence of human/monkey TIMP4 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Metalloproteinase inhibitor 4 antibody
TIMP 4 antibody
TIMP metallopeptidase inhibitor 4 antibody
TIMP-4 antibody
Timp4 antibody
TIMP4_HUMAN antibody
Tissue inhibitor of metalloproteinase 4 antibody
Tissue inhibitor of metalloproteinases 4 antibody