Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Usd: 1219 Special Discount

Specification

96T

Bulk Order Quote | Customization Request

Catalog# EHY0240

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Application

ELISA

Reactivity

Human

Standard curve range

7.81~500 ng/ml

Overview

Product Name

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Product Type

Elisa Kit

Sensitivity

0.13 ng/ml (10 μl)

ng/ml	O.D.		Average	Corrected
0.00	0.0067	0.0074	0.0071
7.81	0.0653	0.0632	0.0643	0.0572
15.63	0.1304	0.1285	0.1295	0.1224
31.25	0.2518	0.2511	0.2515	0.2444
62.50	0.5190	0.4830	0.5010	0.4940
125.00	1.0650	1.0140	1.0395	1.0325
250.00	2.1670	2.0600	2.1135	2.1065
500.00	3.8530	3.7760	3.8145	3.8075

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (ng/ml)	8.7	45.4	146.7	9.0	45.9	148.6
Standard Deviation	0.3	1.1	5.3	0.3	1.5	4.2
Coefficient of Variation (%)	3.4	2.4	3.6	3.2	3.4	2.8

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of Human/Monkey PCSK9 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 115% with an overall mean recovery of 103%.

Sample Value

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Human	30	40.43-115.83	100	78.34
Monkey	6	7.30-197.99	100	93.20

Serum/Plasma – sample from apparently healthy volunteer/monkey were evaluated for the presence of Human/Monkey PCSK9 in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

60 min

Standard Curve Gradient

7 Points

Incubation Count

Detectable Sample

Dosing Volume

10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

Function

Proprotein convertase subtilisin kexin 9 (PCSK9), also named neural apoptosis-regulated convertase 1 (NARC-1), is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. The full-length protein has 692 amino acids, including a signal peptide, a pro- domain, and a catalytic domain. PCSK9 is highly expressed in the liver, intestine, and kidney. It is initially synthesized as a soluble 74 kDa precursor protein. In the endoplasmic reticulum, it undergoes autocatalytic intramolecular cleavage to generate a 14 kDa pro- domain and a 60 kDa catalytic domain. These two domains remain associated when PCSK9 is secreted outside the cells. The primary physiologic function of PCSK9 is to mediate the degradation of low density lipoprotein receptor (LDL R). Early observations indicated that gain-of-function missense mutations in the PCSK9 gene can cause an autosomal dominant form of hypercholesterolemia. The expression of PCSK9 was observed to be up-regulated by the sterol regulatory element binding proteins (SREBPs), a family of transcription factors that are responsible for the upregulation of genes involved in cholesterol and fatty acid metabolism, such as the LDL R gene. Further experimental evidence revealed that in mice, when the PCSK9 gene was knocked out, the number of LDL R in hepatocytes increased, whereas when PCSK9 was over-expressed, the amount of LDL R protein was reduced in the liver. In humans, genetic analyses have shown that individuals who have nonsense or loss-of-function mutations in the PCSK9 gene have significantly lower plasma LDL cholesterol levels. These investigations clearly indicated that PCSK9 plays a key role in reducing the hepatic LDL R levels. Recently, the underlying mechanism has been uncovered: under normal physiologic conditions, the LDL R is internalized on the cell surface and directed to the endosomes in order to be recycled back to the cell surface. PCSK9 binds to the EGF domain of the LDL R and prevents LDL R from being sorted to the endosomes. Instead, the PCSK9/LDL R complex is redistributed to the lysosomes for degradation. As such, PCSK9 regulates the amount of LDL R in the circulation and modulates cholesterol levels. Serum PCSK9 concentrations have been found to be directly associated with cholesterol levels. Since individuals with loss-of-function PCSK9 mutations have strikingly reduced risk of coronary heart diseases, PCSK9 has become an attractive drug target in recent years. One approach is to generate small molecules that are able to interfere with PCSK9 autoactivation and its interaction with LDL R. Other approaches aiming to reduce the amounts of PCSK9 in the circulation, such as small interfering RNAs (siRNAs), have also shown promise.

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Usd: 1219 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

Function

Same Target and Pathway Products

Human IFN-β enzyme-linked immunoassay kit

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD163 enzyme-linked immunoassay kit

Human Renin enzyme-linked immunoassay kit

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)

Lentivirus HIV-1 p24 enzyme-linked immunoassay kit（one step）

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human SNAP25 enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human CXCL2/MIP2/GRO beta enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human IL-3 enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human B7-H6/NCR3LG1 enzyme-linked immunoassay kit

Human CD48 enzyme-linked immunoassay kit

Human MMP-7 enzyme-linked immunoassay kit

Human CTLA-4 enzyme-linked immunoassay kit

Human/Monkey/Canine CCL5/RANTES enzyme-linked immunoassay kit(one step)

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit（one step）

Human CCL2/MCP-1 enzyme-linked immunoassay kit

Human CCL5/RANTES enzyme-linked immunoassay kit

Human FGF-19 enzyme-linked immunoassay kit

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human CD73 enzyme-linked immunoassay kit

Human CD47 enzyme-linked immunoassay kit

Human Neuropilin-1/NRP1 enzyme-linked immunoassay kit（one step）

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human IL-13R alpha 2 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human RBP4 enzyme-linked immunoassay kit（one step）

Human Caspase-9 enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human TGF-α enzyme-linked immunoassay kit

Human Leptin enzyme-linked immunoassay kit

Human CD27/TNFRSF7 enzyme-linked immunoassay kit

Human MERTK enzyme-linked immunoassay kit（one step）

Human ICAM-1 / CD54 enzyme-linked immunoassay kit

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human Endoglin/CD105 enzyme-linked immunoassay kit

Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit

Human beta-NGF enzyme-linked immunoassay kit

Human IL-15 enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human CD28 enzyme-linked immunoassay kit

Human CCL25 enzyme-linked immunoassay kit

Human Growth Hormone enzyme-linked immunoassay kit

Human Siglec 9 enzyme-linked immunoassay kit