Human/Monkey Lipocalin-2/NGAL enzyme-linked immunoassay kit（one step）
Catalog# EHY0166
Human/Monkey Lipocalin-2/NGAL enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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0.14~100 ng/ml
Overview
Product Name
Human/Monkey Lipocalin-2/NGAL enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.01 ng/ml (50μl)；0.03 ng/ml (10μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0126 | 0.0115 | 0.0121 | |
| 0.14 | 0.0241 | 0.0241 | 0.0241 | 0.0121 |
| 0.41 | 0.0450 | 0.0408 | 0.0429 | 0.0309 |
| 1.23 | 0.0977 | 0.0952 | 0.0965 | 0.0844 |
| 3.70 | 0.2584 | 0.2660 | 0.2622 | 0.2502 |
| 11.11 | 0.7241 | 0.7346 | 0.7294 | 0.7173 |
| 33.33 | 1.8220 | 1.8880 | 1.8550 | 1.8430 |
| 100.00 | 3.2360 | 3.2210 | 3.2285 | 3.2165 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 2.3 | 12.0 | 41.8 | 1.7 | 9.7 | 38.1 |
| Standard Deviation | 0.1 | 0.3 | 1.8 | 0.1 | 0.5 | 1.3 |
| Coefficient of Variation (%) | 4.7 | 2.7 | 4.3 | 4.5 | 4.8 | 3.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Lipocalin-2/NGAL，未加人 Lipocalin-2/NGAL 的血清作为本底，计算回收率。回收率的范围从 92 % 至 105 %，平均回收率为 97 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 11.47-84.79 | 100 | 49.12 |
应用本试剂盒，检测30份健康人和2份健康猴的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
24p3
25 kDa alpha 2 microglobulin related subunit of MMP9
25 kDa alpha-2-microglobulin-related subunit of MMP-9
Alpha 2 microglobulin related protein
HGNC:6526
HNL
Lcn 2
Lcn2
Lipocalin-2
Migration stimulating factor inhibitor
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