Human/Monkey VEGFR3/FLT4 enzyme-linked immunoassay kit(one step)
Catalog# EHY0276
Human/Monkey VEGFR3/FLT4 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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Monkey
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7.81~500 ng/ml
Overview
Product Name
Human/Monkey VEGFR3/FLT4 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.10 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0035 | 0.0032 | 0.0032 | |
| 7.81 | 0.0605 | 0.0614 | 0.0610 | 0.0578 |
| 15.63 | 0.1381 | 0.1410 | 0.1396 | 0.1364 |
| 31.25 | 0.2995 | 0.2867 | 0.2931 | 0.2899 |
| 62.50 | 0.5641 | 0.6069 | 0.5855 | 0.5823 |
| 125.00 | 1.0540 | 1.0820 | 1.0680 | 1.0648 |
| 250.00 | 1.7850 | 1.6480 | 1.7165 | 1.7133 |
| 500.00 | 2.4030 | 2.4070 | 2.4050 | 2.4018 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 7.3 | 38.1 | 128.0 | 8.3 | 40.4 | 128.0 |
| Standard Deviation | 0.3 | 1.8 | 6.3 | 0.4 | 1.8 | 6.1 |
| Coefficient of Variation (%) | 4.6 | 4.7 | 4.9 | 4.9 | 4.5 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human/monkey VEGFR3/FLT4 into health human/monkey serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 91% to 117% with an overall mean recovery of 101%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 26.62-155.63 | 100 | 101.03 |
| Monkey Serum | 2 | 100.08-251.00 | 100 | 175.54 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and two samples from healthy monkeys were evaluated for the presence of human/monkey VEGFR3/FLT4 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃