Human/Monkey ESM1 enzyme-linked immunoassay kit

UNIPROT

SwissProt: Q9NQ30 Human

Alias

Endocan antibody

Endothelial cell specific molecule 1 antibody

Endothelial cell-specific molecule 1 antibody

ESM 1 antibody

ESM 1 secretory protein antibody

ESM-1 antibody

Esm1 antibody

ESM1 secretory protein antibody

ESM1_HUMAN antibody

Function

Endocan (endothelial cell proteoglycan; also known as endothelial-cell specific molecule-1 [ESM-1]), is a 50 kDa, monomeric, secreted, cysteine-rich proteoglycan identified initially in endothelial cells of the kidney and lung. Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region. The N-terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N-linked glycosylation sites. Based on human Endocan, there are at least two potential O-linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule. The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4-O sulfated N-acetyl galactosamine with alpha -iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity. Mature mouse Endocan is 96% and 74% aa identical to rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa’s 82 through 131, a range which would not remove the dermatan sulfate attachment site. It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium. It is upregulated by TNF-alpha and VEGF,and is known to bind to LFA-1 ( alpha L beta 2) on the surface of PBMCs, blocking LFA-1 interaction with ICAM-1. Normal circulating levels of Endocan are approximately 1 ng/mL.