PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81] - BSA and Azide free
Catalog# HA750661
PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81] - BSA and Azide free
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WB
-
Human
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HA721510
含抗保成分
-
unconjugated
Overview
Product Name
PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PERK aa 51-450 / 1,116.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 125 kDa
Positive Control
MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, U-87 MG cell lysate, K-562 cell lysate.
Conjugation
unconjugated
Clone Number
PSH0-81
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Eukaryotic translation initiation factor 2-alpha kinase 3, also known as protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), is an enzyme that in humans is encoded by the EIF2AK3 gene. The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Patients with mutations in this gene develop Wolcott-Rallison syndrome. EIF2AK3 has been shown to interact with DNAJC3, NFE2L2, and endoplasmic reticulum chaperone BiP (Hsp70).
Background References
1. Zhang D et al. A non-canonical cGAS-STING-PERK pathway facilitates the translational program critical for senescence and organ fibrosis. Nat Cell Biol. 2022 May
2. Raines LN et al. PERK is a critical metabolic hub for immunosuppressive function in macrophages. Nat Immunol. 2022 Mar
Subcellular Location
Endoplasmic reticulum membrane.
UNIPROT
Synonyms
DKFZp781H1925 antibody
E2AK3_HUMAN antibody
EC 2.7.11.1 antibody
Eif2ak3 antibody
Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
Eukaryotic translation initiation factor 2-alpha kinase 3 antibody
Heme regulated EIF2 alpha kinase antibody
HRI antibody
HsPEK antibody
Pancreatic eIF2 alpha kinase antibody
ExpandImages
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Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (HA750661) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: A549 cell lysate (20 µg/Lane)
Lane 6: U-87 MG cell lysate (20 µg/Lane)
Lane 7: K-562 cell lysate (16 µg/Lane)
Predicted band size: 125 kDa
Observed band size: 140 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750661) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
PERK Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81]
Application: WB
Reactivity: Human
Conjugate: unconjugated
