PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81] (HA721510)
Catalog# HA721510
Rabbit Monoclonal to PERK
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WB
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Human
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: A549 cell lysate (20 µg/Lane)
Lane 6: U-87 MG cell lysate (20 µg/Lane)
Lane 7: K-562 cell lysate (16 µg/Lane)
Predicted band size: 125 kDa
Observed band size: 140 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721510) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81] (HA721510)
Immunogen
Recombinant protein within human perk aa 51-450 / 1,116.
Host
Rabbit
Positive Control
MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, U-87 MG cell lysate, K-562 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH0-81
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 125 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PERK
SYNONYMS
DKFZp781H1925 antibody;E2AK3_HUMAN antibody;EC 2.7.11.1 antibody;Eif2ak3 antibody;Eukaryotic translation initiation factor 2 alpha kinase 3 antibody;Eukaryotic translation initiation factor 2-alpha kinase 3 antibody;Heme regulated EIF2 alpha kinase antibody;HRI antibody;HsPEK antibody;Pancreatic eIF2 alpha kinase antibody;Pancreatic eIF2-alpha kinase antibody;PEK antibody;PRKR like endoplasmic reticulum kinase antibody;PRKR-like endoplasmic reticulum kinase antibody;WRS antibody
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane.
FUNCTION
Eukaryotic translation initiation factor 2-alpha kinase 3, also known as protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), is an enzyme that in humans is encoded by the EIF2AK3 gene. The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Patients with mutations in this gene develop Wolcott-Rallison syndrome. EIF2AK3 has been shown to interact with DNAJC3, NFE2L2, and endoplasmic reticulum chaperone BiP (Hsp70).
CITATIONS
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Gankun Yuan,et al
Novel immunogenic cell death inducer combined with autophagy inhibitor to amplify photodynamic synergistic immunotherapy for triple-negative breast cancer
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WB
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Citation
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