PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81]
Overview
Product Name
PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PERK aa 51-450 / 1,116.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 125 kDa
Positive Control
MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, U-87 MG cell lysate, K-562 cell lysate.
Conjugation
unconjugated
Clone Number
PSH0-81
RRID
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Eukaryotic translation initiation factor 2-alpha kinase 3, also known as protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), is an enzyme that in humans is encoded by the EIF2AK3 gene. The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Patients with mutations in this gene develop Wolcott-Rallison syndrome. EIF2AK3 has been shown to interact with DNAJC3, NFE2L2, and endoplasmic reticulum chaperone BiP (Hsp70).
Background References
1. Zhang D et al. A non-canonical cGAS-STING-PERK pathway facilitates the translational program critical for senescence and organ fibrosis. Nat Cell Biol. 2022 May
2. Raines LN et al. PERK is a critical metabolic hub for immunosuppressive function in macrophages. Nat Immunol. 2022 Mar
Subcellular Location
Endoplasmic reticulum membrane.
UNIPROT
Synonyms
DKFZp781H1925 antibody
E2AK3_HUMAN antibody
EC 2.7.11.1 antibody
Eif2ak3 antibody
Eukaryotic translation initiation factor 2 alpha kinase 3 antibody
Eukaryotic translation initiation factor 2-alpha kinase 3 antibody
Heme regulated EIF2 alpha kinase antibody
HRI antibody
HsPEK antibody
Pancreatic eIF2 alpha kinase antibody
ExpandImages
-
Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (HA721510) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: A549 cell lysate (20 µg/Lane)
Lane 6: U-87 MG cell lysate (20 µg/Lane)
Lane 7: K-562 cell lysate (16 µg/Lane)
Predicted band size: 125 kDa
Observed band size: 140 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721510) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI: 10.1016/j.ecoenv.2025.119006
IF: 6.1
Application: WB
Reactivity: Human
Publish date: 2025 Sept
-
Ethanol-induced liver injury is alleviated by chrysin via increasing MRP2 transporter expression and enhancing the Nrf2-mediated adaptive response
Journal: Naunyn-Schmiedebergs Archives Of Pharmacology
DOI: 10.1007/s00210-025-04774-9
IF: 3.1
Application: WB
Reactivity: Rat
Publish date: 2025 Oct
-
Sappanchalcone Suppresses NSCLC by Oxidative Stress-Driven DNA Damage and ER Stress Activation through PIEZO1 Modulation
Journal: iScience
DOI: 10.1016/j.isci.2025.114057
IF: 4.1
Application: WB
Reactivity: Human
Publish date: 2025 Nov
-
Endoplasmic Reticulum-Targeted Photodynamic Therapy Combined with Autophagy Inhibition to Enhance Synergistic Immunotherapy for Triple-Negative Breast Cancer
Journal: Chemistry - A European Journal
DOI: 10.1002/chem.202500146
IF: 3.9
Application:
Reactivity:
Publish date: 2025 May
-
Novel immunogenic cell death inducer combined with autophagy inhibitor to amplify photodynamic synergistic immunotherapy for triple-negative breast cancer
Journal: Research Square
DOI: 10.21203/rs.3.rs-4950334/v1
IF:
Application: WB
Reactivity: Mouse
Publish date: 2024 Oct
Products with the same target and pathway
PERK Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated
PERK Recombinant Rabbit Monoclonal Antibody [PSH0-81] - BSA and Azide free
Application: WB
Reactivity: Human
Conjugate: unconjugated
