Phospho-IRE1 (S724) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-35] (HA750869)
Catalog# HA750869
Rabbit Monoclonal to Phospho-IRE1 (S724)
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-IRE1 (S724) on different lysates with Rabbit anti-Phospho-IRE1 (S724) antibody (HA750869) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane)
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 5 minutes 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750869) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-IRE1 (S724) on different lysates with Rabbit anti-Phospho-IRE1 (S724) antibody (HA750869) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane)
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 5 minutes 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750869) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.