Phospho-IRE1 (S724) Recombinant Rabbit Monoclonal Antibody [PSH03-35] (HA721980)
Catalog# HA721980
Rabbit Monoclonal to Phospho-IRE1 (S724)
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
-
Mouse
Lane 1: HeLa cell lysate
Lane 2: HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721980) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-IRE1 (S724) Recombinant Rabbit Monoclonal Antibody [PSH03-35] (HA721980)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser724 of human ire1.
Host
Rabbit
Modification
Phospho
Modification Site
S724
Positive Control
HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate, Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, Jurkat cells treated with 100nM Calyculin A for 30 minutes, human pancreas tissue, HeLa cells treated with 100nM Calyculin A for 30 minutes.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH03-35
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
-
IF-Cell
-
1:500
-
IHC-P
-
1:500
-
FC
-
1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 110 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-IRE1 (S724)
SYNONYMS
Endoplasmic reticulum (ER) to nucleus signalling 1 antibody;Endoplasmic reticulum to nucleus signaling 1 antibody;Endoplasmic reticulum-to-nucleus signaling 1 antibody;Endoribonuclease antibody;ER to nucleus signaling 1 antibody;ERN 1 antibody;Ern1 antibody;ERN1_HUMAN antibody;hIRE 1p antibody;hIRE1p antibody;Inositol requiring 1 antibody;Inositol requiring 1, S. cerevisiae, homolog of antibody;Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody;Inositol requiring protein 1 antibody;inositol-requiring enzyme 1 antibody;Inositol-requiring protein 1 antibody;IRE 1 antibody;IRE 1a antibody;IRE 1P antibody;Ire1 alpha antibody;Ire1-alpha antibody;IRE1a antibody;Ire1alpha antibody;IRE1P antibody;MGC163277 antibody;MGC163279 antibody;Protein kinase/endoribonuclease antibody;RGD1559716 antibody;Serine/threonine protein kinase/endoribonuclease IRE1 antibody
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane; nucleus.
FUNCTION
The serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 α (IRE1α) is an enzyme that in humans is encoded by the ERN1 gene. The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals (mainly the unfolded protein response). Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.