TOM1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-38] (HA750872)
Catalog# HA750872
Rabbit Monoclonal to TOM1
Tested for
-
WB
-
IF-Tissue
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of TOM1 on different lysates with Rabbit anti-TOM1 antibody (HA750872) at 1/2,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: U-2 OS cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: JAR cell lysate (20 µg/Lane)
Lane 8: THP-1 cell lysate (20 µg/Lane)
Lane 9: C2C12 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse heart tissue lysate (40 µg/Lane)
Lane 12: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 13: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750872) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: U-2 OS cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: JAR cell lysate (20 µg/Lane)
Lane 8: THP-1 cell lysate (20 µg/Lane)
Lane 9: C2C12 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse heart tissue lysate (40 µg/Lane)
Lane 12: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 13: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750872) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.