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Glutathione Peroxidase 3 / GPx-3 Recombinant Rabbit Monoclonal Antibody [PSH17-06] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751612

Glutathione Peroxidase 3 / GPx-3 Recombinant Rabbit Monoclonal Antibody [PSH17-06] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Glutathione Peroxidase 3 / GPx-3 Recombinant Rabbit Monoclonal Antibody [PSH17-06] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 26 kDa

Positive Control

Mouse kidney tissue lysate, Rat kidney tissue lysate, mouse kidney tissue, mouse lung tissue, rat kidney tissue, rat lung tissue.

Conjugation

unconjugated

Clone Number

PSH17-06

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

Target

Function

Glutathione peroxidase 3 (GPx-3), also known as plasma glutathione peroxidase (GPx-P) or extracellular glutathione peroxidase is an enzyme that in humans is encoded by the GPX3 gene. GPx-3 belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. GPx-3 has a wide thiol specificity. The sources of reducing power for GPx-3 in vitro include GSH, cysteine, mercaptoethanol, and dithiothreitol. There is an evidence of effectiveness of homocysteine in reduction of GPx-3: GSH can be completely replaced by reduced homocysteine in vitro.

Background References

1. Li Y et al. Glutathione Peroxidase 3 induced mitochondria-mediated apoptosis via AMPK /ERK1/2 pathway and resisted autophagy-related ferroptosis via AMPK/mTOR pathway in hyperplastic prostate. J Transl Med. 2023 Aug

2. Song Y et al. Glutathione peroxidase 3 is essential for countering senescence in adipose remodelling by maintaining mitochondrial homeostasis. Redox Biol. 2024 Nov

Subcellular Location

Secreted.

UNIPROT

SwissProt: P46412 Mouse

SwissProt: P23764 Rat

Synonyms

Extracellular glutathione peroxidase antibody

Glutathione peroxidase 3 (plasma) antibody

Glutathione peroxidase 3 antibody

GPX 3 antibody

GPx P antibody

GPx-3 antibody

GPx-P antibody

GPX3 antibody

GPXP antibody

GSHPx 3 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Glutathione Peroxidase 3 / GPx-3 on different lysates with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)<br />Lane 3: Rat kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 26 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Glutathione Peroxidase 3 / GPx-3 on different lysates with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (HA751612) at 1/5,000 dilution.

    Lane 1: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)
    Lane 3: Rat kidney tissue lysate (40 µg/Lane)

    Predicted band size: 26 kDa
    Observed band size: 22 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751612) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (HA751612) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751612) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (HA751612) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751612) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (HA751612) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751612) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751612" style="font-weight: bold;text-decoration: underline;">HA751612</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Glutathione Peroxidase 3 / GPx-3 antibody (HA751612) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751612) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"