Biotin Conjugated Human Alpha-Synuclein Recombinant Rabbit Monoclonal Antibody [PSH12-56] - Detector
Overview
Product Name
Biotin Conjugated Human Alpha-Synuclein Recombinant Rabbit Monoclonal Antibody [PSH12-56] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Alpha-Synuclein aa 1-140.
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Alpha-Synuclein protein (HA211150).
Conjugation
Biotin
Clone Number
PSH12-56
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [JB42-33] to Human Alpha-Synuclein antibody (Capture) (HA723467) and Recombinant Human Alpha-Synuclein protein (HA211150) as the standard. The reference range value is 313-20,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Alpha-synuclein (aSyn) is a protein that, in humans, is encoded by the SNCA gene. Alpha-synuclein is a neuronal protein that regulates synaptic vesicle trafficking and subsequent neurotransmitter release. It is abundant in the brain, while smaller amounts are found in the heart, muscle and other tissues. In the brain, alpha-synuclein is found mainly in the axon terminals of presynaptic neurons. Within these terminals, alpha-synuclein interacts with phospholipids and proteins. Presynaptic terminals release chemical messengers, called neurotransmitters, from compartments known as synaptic vesicles. The release of neurotransmitters relays signals between neurons and is critical for normal brain function. In Parkinson's disease and other synucleinopathies, insoluble forms of alpha-synuclein accumulate as inclusions in Lewy bodies. Familial Parkinson's disease is associated with mutations in the -synuclein (SNCA) gene. In the process of seeded nucleation, alpha-synuclein acquires a cross-sheet structure similar to other amyloids.
Background References
1. Prieto Huarcaya S et al. Recombinant pro-CTSD (cathepsin D) enhances SNCA/alpha-Synuclein degradation in alpha-Synucleinopathy models. Autophagy. 2022 May
2. Li R et al. Piperine promotes autophagy flux by P2RX4 activation in SNCA/alpha-synuclein-induced Parkinson disease model. Autophagy. 2022 Mar
Subcellular Location
Cytoplasm, Membrane, Nucleus, Synapse, Secreted, Cell projection, axon.
UNIPROT
Synonyms
Alpha synuclein antibody
Alpha-synuclein antibody
Alpha-synuclein, isoform NACP140 antibody
alphaSYN antibody
MGC105443 antibody
MGC110988 antibody
MGC127560 antibody
MGC64356 antibody
NACP antibody
Non A beta component of AD amyloid antibody
ExpandImages
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![Sandwich ELISA analysis of Human Alpha-Synuclein matched pair antibodies<br /><br />Capture: <a href="/products/HA723467" style="font-weight: bold;text-decoration: underline;">HA723467</a>, Human Alpha-Synuclein Rabbit mAb [JB42-33]<br />Detector: <a href="/products/HA723468" style="font-weight: bold;text-decoration: underline;">HA723468</a>, Human Alpha-Synuclein Rabbit mAb [PSH12-56]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA723467" style="font-weight: bold;text-decoration: underline;">HA723467</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Alpha-synuclein protein (<a href="/products/HA211150" style="font-weight: bold;text-decoration: underline;">HA211150</a>) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723468" style="font-weight: bold;text-decoration: underline;">HA723468</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723469B_1.png?v=20260401002302)
Sandwich ELISA analysis of Human Alpha-Synuclein matched pair antibodies
Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33]
Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723467) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human Alpha-synuclein protein (HA211150) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA723468, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native alpha-Synuclein in SH-SY5Y extract samples based on a 1,000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA723467" style="font-weight: bold;text-decoration: underline;">HA723467</a>, Human Alpha-Synuclein Rabbit mAb [JB42-33]<br />Detector: <a href="/products/HA723468" style="font-weight: bold;text-decoration: underline;">HA723468</a>, Human Alpha-Synuclein Rabbit mAb [PSH12-56]<br /><br />The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 2,195 pg/ml in SH-SY5Y extract.](https://storage.huabio.cn/huabio/productImg/HA723469B_2.png?v=20260401002302)
Interpolated concentrations of native alpha-Synuclein in SH-SY5Y extract samples based on a 1,000 µg/ml extract load.
Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33]
Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56]
The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are SH-SY5Y extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 2,195 pg/ml in SH-SY5Y extract. -
![Interpolated concentrations of native alpha-Synuclein in human serum samples.<br /><br />Capture: <a href="/products/HA723467" style="font-weight: bold;text-decoration: underline;">HA723467</a>, Human Alpha-Synuclein Rabbit mAb [JB42-33]<br />Detector: <a href="/products/HA723468" style="font-weight: bold;text-decoration: underline;">HA723468</a>, Human Alpha-Synuclein Rabbit mAb [PSH12-56]<br /><br />The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 21.2 ng/ml in human serum.](https://storage.huabio.cn/huabio/productImg/HA723469B_3.png?v=20260401002302)
Interpolated concentrations of native alpha-Synuclein in human serum samples.
Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33]
Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56]
The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-Synuclein concentration was determined to be 21.2 ng/ml in human serum. -
![Interpolated concentrations of spiked alpha-Synuclein in human cell culture media samples.<br /><br />Capture: <a href="/products/HA723467" style="font-weight: bold;text-decoration: underline;">HA723467</a>, Human Alpha-Synuclein Rabbit mAb [JB42-33]<br />Detector: <a href="/products/HA723468" style="font-weight: bold;text-decoration: underline;">HA723468</a>, Human Alpha-Synuclein Rabbit mAb [PSH12-56]<br /><br />The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723469B_4.png?v=20260401002302)
Interpolated concentrations of spiked alpha-Synuclein in human cell culture media samples.
Capture: HA723467, Human Alpha-Synuclein Rabbit mAb [JB42-33]
Detector: HA723468, Human Alpha-Synuclein Rabbit mAb [PSH12-56]
The concentrations of alpha-Synuclein were measured in duplicates, interpolated from the alpha-Synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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