Human/Mouse IL-20 enzyme-linked immunoassay kit
Catalog# EH0275
Human/Mouse IL-20 enzyme-linked immunoassay kit
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ELISA
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Human
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Mouse
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7.81~500 pg/ml
Overview
Product Name
Human/Mouse IL-20 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.25 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0434 | 0.0467 | 0.0451 | |
| 7.81 | 0.1143 | 0.1205 | 0.1174 | 0.0724 |
| 15.63 | 0.1864 | 0.1869 | 0.1867 | 0.1416 |
| 31.25 | 0.3266 | 0.3311 | 0.3289 | 0.2838 |
| 62.50 | 0.5885 | 0.5804 | 0.5845 | 0.5394 |
| 125.00 | 1.0450 | 1.0350 | 1.0400 | 0.9950 |
| 250.00 | 1.7580 | 1.7400 | 1.7490 | 1.7040 |
| 500.00 | 2.9120 | 2.9250 | 2.9185 | 2.8735 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 26.4 | 121.0 | 357.4 | 25.4 | 120.0 | 348.5 |
| Standard Deviation | 0.8 | 4.4 | 16.4 | 1.1 | 4.0 | 6.6 |
| Coefficient of Variation (%) | 3.1 | 3.6 | 4.6 | 4.5 | 3.3 | 1.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human/mouse IL-20 into health human/mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 97%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human serum | 30 | n.d. | 0 | n.d. |
| Mouse serum | 30 | 7.43-60.30 | 100 | 23.29 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and thirty samples from apparently healthy mice were evaluated for the presence of human/mouse IL-20 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃