Human/Mouse MASP2 enzyme-linked immunoassay kit
Catalog# EH0231
Human/Mouse MASP2 enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
Overview
Product Name
Human/Mouse MASP2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.65 pg/ml (50 μl)；14.43 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0088 | 0.0087 | 0.0088 | |
| 156.25 | 0.1134 | 0.1133 | 0.1134 | 0.1046 |
| 312.50 | 0.2231 | 0.2098 | 0.2165 | 0.2077 |
| 625.00 | 0.4160 | 0.4043 | 0.4102 | 0.4014 |
| 1250.00 | 0.7754 | 0.7621 | 0.7688 | 0.7600 |
| 2500.00 | 1.4450 | 1.3370 | 1.3910 | 1.3823 |
| 5000.00 | 2.3410 | 2.3980 | 2.3695 | 2.3608 |
| 10000.00 | 3.4990 | 3.5180 | 3.5085 | 3.4998 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 201.2 | 968.9 | 3123.1 | 197.1 | 1011.0 | 3287.9 |
| Standard Deviation | 9.5 | 46.0 | 104.7 | 8.3 | 47.6 | 158.0 |
| Coefficient of Variation (%) | 4.7 | 4.7 | 3.4 | 4.2 | 4.7 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human/mouse MASP2 into health human/mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 120% with an overall mean recovery of 101%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.58-41.69 | 100 | 23.31 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of MASP2 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Mannan binding lectin serine peptidase 2
Mannan binding lectin serine protease 2
Mannose binding protein associated serine protease 2
MAP 19
MAP19
MASP 2
MBL associated plasma protein of 19 kD
MBL associated serine protease 2
Small MBL associated protein
sMAP