Human/Mouse MASP2 enzyme-linked immunoassay kit
Catalog# EH0231
Human/Mouse MASP2 enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
Overview
Product Name
Human/Mouse MASP2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.65 pg/ml (50 μl)；14.43 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0088 | 0.0087 | 0.0088 | |
| 156.25 | 0.1134 | 0.1133 | 0.1134 | 0.1046 |
| 312.50 | 0.2231 | 0.2098 | 0.2165 | 0.2077 |
| 625.00 | 0.4160 | 0.4043 | 0.4102 | 0.4014 |
| 1250.00 | 0.7754 | 0.7621 | 0.7688 | 0.7600 |
| 2500.00 | 1.4450 | 1.3370 | 1.3910 | 1.3823 |
| 5000.00 | 2.3410 | 2.3980 | 2.3695 | 2.3608 |
| 10000.00 | 3.4990 | 3.5180 | 3.5085 | 3.4998 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 201.2 | 968.9 | 3123.1 | 197.1 | 1011.0 | 3287.9 |
| Standard Deviation | 9.5 | 46.0 | 104.7 | 8.3 | 47.6 | 158.0 |
| Coefficient of Variation (%) | 4.7 | 4.7 | 3.4 | 4.2 | 4.7 | 4.8 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人/小鼠MASP2，未加人/小鼠 MASP2 的血清作为本底，计算回收率。回收率的范围从 81 % 至 120 %，平均回收率为 101 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.58-41.69 | 100 | 23.31 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Mannan binding lectin serine peptidase 2
Mannan binding lectin serine protease 2
Mannose binding protein associated serine protease 2
MAP 19
MAP19
MASP 2
MBL associated plasma protein of 19 kD
MBL associated serine protease 2
Small MBL associated protein
sMAP