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FOXP3 Recombinant Rabbit Monoclonal Antibody

Usd: 209

Specification

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Catalog# IRS008

FOXP3 Recombinant Rabbit Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

FOXP3 Recombinant Rabbit Monoclonal Antibody

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human FOXP3 aa 101-370.

Species Reactivity

Human

Validated Applications

mIHC

Target Molecular Weight

Predicted band size: 47 kDa

Positive Control

Human lung cancer tissue, human tonsil tissue, human lung squamous cell carcinoma tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100

Target

Function

FOXP3 (forkhead box P3), also known as scurfin, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells. Regulatory T cells generally turn the immune response down. In cancer, an excess of regulatory T cell activity can prevent the immune system from destroying cancer cells. In autoimmune disease, a deficiency of regulatory T cell activity can allow other autoimmune cells to attack the body's own tissues.

Background References

1. Wang J et al. Human FOXP3 and tumour microenvironment. Immunology. 2023 Feb

2. Ziółkowska-Suchanek I et al. FOXP3: A Player of Immunogenetic Architecture in Lung Cancer. Genes (Basel). 2024 Apr

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9BZS1 Human

Synonyms

AIID antibody

DIETER antibody

Forkhead box P3 antibody

Forkhead box protein P3 antibody

FOXP3 antibody

FOXP3_HUMAN antibody

FOXP3delta7 antibody

Immune dysregulation polyendocrinopathy enteropathy X linked antibody

Immunodeficiency polyendocrinopathy enteropathy X linked antibody

IPEX antibody

Expand

Images

  • mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with FOXP3 (<a href="/products/IRS008" style="font-weight: bold;text-decoration: underline;">IRS008</a>), CD57 (<a href="/products/IRS027" style="font-weight: bold;text-decoration: underline;">IRS027</a>), MPO (<a href="/products/IRS037" style="font-weight: bold;text-decoration: underline;">IRS037</a>), CD8 (<a href="/products/IRS007" style="font-weight: bold;text-decoration: underline;">IRS007</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with FOXP3 (IRS008), CD57 (IRS027), MPO (IRS037), CD8 (IRS007) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (<a href="/products/IRS008" style="font-weight: bold;text-decoration: underline;">IRS008</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (IRS008) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human lung squamous cell carcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (<a href="/products/IRS008" style="font-weight: bold;text-decoration: underline;">IRS008</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human lung squamous cell carcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (IRS008) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"