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FOXP3 Recombinant Rabbit Monoclonal Antibody

Usd: 385

Specification

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Catalog# IRS008

FOXP3 Recombinant Rabbit Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

FOXP3 Recombinant Rabbit Monoclonal Antibody

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human FOXP3 aa 101-370.

Species Reactivity

Human

Validated Applications

mIHC

Molecular Weight

Predicted band size: 47 kDa

Positive Control

Human lung cancer tissue, human tonsil tissue, human lung squamous cell carcinoma tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100

Target

Function

FOXP3 (forkhead box P3), also known as scurfin, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells. Regulatory T cells generally turn the immune response down. In cancer, an excess of regulatory T cell activity can prevent the immune system from destroying cancer cells. In autoimmune disease, a deficiency of regulatory T cell activity can allow other autoimmune cells to attack the body's own tissues.

Background References

1. Wang J et al. Human FOXP3 and tumour microenvironment. Immunology. 2023 Feb

2. Ziółkowska-Suchanek I et al. FOXP3: A Player of Immunogenetic Architecture in Lung Cancer. Genes (Basel). 2024 Apr

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9BZS1 Human

Synonyms

AIID antibody

DIETER antibody

Forkhead box P3 antibody

Forkhead box protein P3 antibody

FOXP3 antibody

FOXP3_HUMAN antibody

FOXP3delta7 antibody

Immune dysregulation polyendocrinopathy enteropathy X linked antibody

Immunodeficiency polyendocrinopathy enteropathy X linked antibody

IPEX antibody

Expand

Images

  • mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with FOXP3 (<a href="/products/IRS008" style="font-weight: bold;text-decoration: underline;">IRS008</a>), CD57 (<a href="/products/IRS027" style="font-weight: bold;text-decoration: underline;">IRS027</a>), MPO (<a href="/products/IRS037" style="font-weight: bold;text-decoration: underline;">IRS037</a>), CD8 (<a href="/products/IRS007" style="font-weight: bold;text-decoration: underline;">IRS007</a>) and panCK (<a href="/products/IRS010" style="font-weight: bold;text-decoration: underline;">IRS010</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with FOXP3 (IRS008), CD57 (IRS027), MPO (IRS037), CD8 (IRS007) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (<a href="/products/IRS008" style="font-weight: bold;text-decoration: underline;">IRS008</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (IRS008) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of human lung squamous cell carcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (<a href="/products/IRS008" style="font-weight: bold;text-decoration: underline;">IRS008</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of human lung squamous cell carcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-FOXP3 antibody (IRS008) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"