TdT Mouse Monoclonal Antibody [B4-F5]

Function

Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase expressed in immature lymphocytes of the thymus and bone marrow, as well as certain leukemic cells. The nuclear enzyme TdT plays a critical role in generating immune receptor diversity. During rearrangement of Ig and TCR V region genes, TdT catalyzes the addition of N nucleotides without a DNA template to V-D and D-J junctions in both Ig and TCR as well as the V-J junctions in the TCR (1–3). During the fetal and newborn period, TdT activity is absent, resulting in an N region-deficient Ag receptor repertoire significantly lacking in the diversity observed in the junctional regions of Igs and TCRs of adult animals

Background References

1. Yamashita N., Shimazaki N., Ibe S., Kaneko R., Tanabe A., Toyomoto T., Fujita K., Hasegawa T., Toji S., Tamai K., Yamamoto H., Koiwai O.;"Terminal deoxynucleotidyltransferase directly interacts with a novel nuclear protein that is homologous to p65."; Genes Cells 6:641-652(2001).

2. Yang B., Gathy K.N., Coleman M.S.; "Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase."; J. Biol. Chem. 269:11859-11868(1994).

3. Peterson R.C., Cheung L.C., Mattaliano R.J., Chang L.M.S., Bollum F.J.; "Molecular cloning of human terminal deoxynucleotidyltransferase."; Proc. Natl. Acad. Sci. U.S.A. 81:4363-4367(1984).

Sequence Similarity

Belongs to the DNA polymerase type-X family.

Subcellular Location

Cell nucleus

UNIPROT

SwissProt: P04053 Human

Synonyms

Deoxynucleotidyltransferase terminal antibody

DNA nucleotidylexotransferase antibody

DNTT antibody

Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody

TDT antibody

TDT_HUMAN antibody

Terminal addition enzyme antibody

Terminal deoxynucleotidyltransferase antibody

Terminal deoxyribonucleotidyltransferase antibody

Terminal transferase antibody