TdT Mouse Monoclonal Antibody [B4-F5]
Catalog# M0807-13
TdT Mouse Monoclonal Antibody [B4-F5]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
TdT Mouse Monoclonal Antibody [B4-F5]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide (KLH-coupled) corresponding to near C-terminal residues of human TdT.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
58kDa
Positive Control
Jurkat, Hela
Conjugation
unconjugated
Clone Number
B4-F5
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
Target
Function
Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase expressed in immature lymphocytes of the thymus and bone marrow, as well as certain leukemic cells. The nuclear enzyme TdT plays a critical role in generating immune receptor diversity. During rearrangement of Ig and TCR V region genes, TdT catalyzes the addition of N nucleotides without a DNA template to V-D and D-J junctions in both Ig and TCR as well as the V-J junctions in the TCR (1–3). During the fetal and newborn period, TdT activity is absent, resulting in an N region-deficient Ag receptor repertoire significantly lacking in the diversity observed in the junctional regions of Igs and TCRs of adult animals
Background References
1. Yamashita N., Shimazaki N., Ibe S., Kaneko R., Tanabe A., Toyomoto T., Fujita K., Hasegawa T., Toji S., Tamai K., Yamamoto H., Koiwai O.;"Terminal deoxynucleotidyltransferase directly interacts with a novel nuclear protein that is homologous to p65."; Genes Cells 6:641-652(2001).
2. Yang B., Gathy K.N., Coleman M.S.; "Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase."; J. Biol. Chem. 269:11859-11868(1994).
3. Peterson R.C., Cheung L.C., Mattaliano R.J., Chang L.M.S., Bollum F.J.; "Molecular cloning of human terminal deoxynucleotidyltransferase."; Proc. Natl. Acad. Sci. U.S.A. 81:4363-4367(1984).
Sequence Similarity
Belongs to the DNA polymerase type-X family.
Subcellular Location
Cell nucleus
UNIPROT
Synonyms
Deoxynucleotidyltransferase terminal antibody
DNA nucleotidylexotransferase antibody
DNTT antibody
Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
TDT antibody
TDT_HUMAN antibody
Terminal addition enzyme antibody
Terminal deoxynucleotidyltransferase antibody
Terminal deoxyribonucleotidyltransferase antibody
Terminal transferase antibody
Images
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Western blot analysis of TdT on different lysates with Rabbit anti-TdT antibody (M0807-13) at 1/2,000 dilution.
Lane 1: Jurkat (Human T lymphocyte cell) cell lysate
Lane 2: HL-60 (Human myeloid leukemia cell) cell lysate
Lane 3: Mouse liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minutes 50seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M0807-13, 1/2,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 58 kDa
Observed band size: 58 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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