GATA4 Mouse Monoclonal Antibody [7-4]
Catalog# M1306-2
GATA4 Mouse Monoclonal Antibody [7-4]
-
WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
GATA4 Mouse Monoclonal Antibody [7-4]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within mouse GATA4 aa 200-400/442.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
A549 cell lysates, human intestine tissue, human colon carcinoma tissue.
Conjugation
unconjugated
Clone Number
7-4
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500
-
IHC-P
-
1:50-1:200
Target
Function
Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase.
Background References
1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004)
2. "Phenotypes with GATA4 or NKX2.5 mutations in familial atrial septal defect."Hirayama-Yamada K., Kamisago M., Akimoto K., Aotsuka H., Nakamura Y., Tomita H., Furutani M., Imamura S., Takao A., Nakazawa M., Matsuoka R.Am. J. Med. Genet. A 135:47-52(2005)
Post-translational Modification
Methylation at Lys-300 attenuates transcriptional activity.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
ASD2 antibody
GATA 4 antibody
GATA binding protein 4 antibody
GATA-binding factor 4 antibody
gata4 antibody
GATA4_HUMAN antibody
MGC126629 antibody
Transcription factor GATA 4 antibody
Transcription factor GATA-4 antibody
Transcription factor GATA4 antibody
ExpandImages
-
Immunohistochemical analysis of paraffin-embedded human intestine tissue using anti-GATA4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1306-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-GATA4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1306-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Western blot analysis of GATA4 on different lysates with Mouse anti-GATA4 antibody (M1306-2) at 1/1,000 dilution.
Lane 1: A549 (Human lung adenocarcinoma cells) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 50 seconds; ECL: K1802
Negative expression of GATA4 protein in K562 cell is consistent with the predicted expression pattern
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M1306-2, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature
Secondary antibody: Goat Anti-Mouse IgG-HRP(HA1006), 1/50,00 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 45 kDa
Observed band size: 50-55 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
GATA4 Recombinant Rabbit Monoclonal Antibody [PSH23-98]
Application: WB,IF-Cell,IHC-P,FC(Intra)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
GATA4 Recombinant Rabbit Monoclonal Antibody [JA11-41]
Application: WB,IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
GATA4 Recombinant Rabbit Monoclonal Antibody [JA11-41]
Application: WB,IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
