Haptoglobin Mouse Monoclonal Antibody [5-D3]
Catalog# M1510-37
Haptoglobin Mouse Monoclonal Antibody [5-D3]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Haptoglobin Mouse Monoclonal Antibody [5-D3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Native Human Haptoglobin protein.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
Human lung tissue lysate, human liver tissue lysate, HepG2, Hela, rat liver tissue, mouse liver tissue, human liver tissue, human lung cancer tissue.
Conjugation
unconjugated
Clone Number
5-D3
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgM
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:50-1:200
Target
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
Background References
1. Yang Q et al. iTRAQ-Based Proteomics Investigation of Aqueous Humor from Patients with Coats\' Disease. PLoS One 11:e0158611 (2016).
2. Tomazic PV et al. Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis. J Allergy Clin Immunol 133(3):741-50 (2014).
Sequence Similarity
Belongs to the peptidase S1 family.
Tissue Specificity
Expressed by the liver and secreted in plasma.
Subcellular Location
Extracellular region, secreted.
UNIPROT
Synonyms
Binding peptide antibody
BP antibody
Haptoglobin alpha chain antibody
Haptoglobin alpha(1S) beta antibody
Haptoglobin alpha(2FS) beta antibody
Haptoglobin beta chain antibody
Haptoglobin, alpha polypeptide antibody
Haptoglobin, beta polypeptide antibody
HP antibody
HP2 ALPHA2 antibody
ExpandImages
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ICC staining Haptoglobin (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Haptoglobin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Haptoglobin antibody. Counter stained with
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.
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Western blot analysis of Haptoglobin on Human plasma lysate with Rabbit anti-Haptoglobin antibody (M1510-37) at 1/4,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: M1510-37, 1/4,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 45.2 kDa
Observed band size: 45 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Haptoglobin Recombinant Rabbit Monoclonal Antibody [JM10-79]
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
Haptoglobin Recombinant Rabbit Monoclonal Antibody [JM10-79]
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human
Conjugate: unconjugated
Haptoglobin Mouse Monoclonal Antibody [5-D3]
Application: WB,IHC-P,ELISA
Reactivity: Human
Conjugate: unconjugated
