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NSUN4 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1706-16

NSUN4 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

NSUN4 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within N-terminal human NSUN4.

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 43 kDa

Positive Control

MCF7 (Human breast cancer cell) cell lysate, A549, HepG2, human liver cancer tissue, human kidney tissue, mouse testis tissue.

Conjugation

unconjugated

RRID

AB_3073425

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4.

Background References

1. Metodiev MD et al.SUN4 is a dual function mitochondrial protein required for both methylation of 12S rRNA and coordination of mitoribosomal assembly.LoS Genet 10(2):e1004110 (2014).

2. Yakubovskaya Et al.tructure of the essential MTERF4:NSUN4 protein complex reveals how an MTERF protein collaborates to facilitate rRNA modification.Structure 20(11):1940-7 (2012).

Sequence Similarity

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: Q96CB9 Human

SwissProt: Q9CZ57 Mouse

Synonyms

5 methylcytosine rRNA methyltransferase NSUN4 antibody

EC=2.1.1.- antibody

NOL1/NOP2/Sun domain family member 4 antibody

NOL1/NOP2/Sun domain family, member 4 antibody

NOP2/Sun domain family, member 4 antibody

Nsun4 antibody

NSUN4_HUMAN antibody

Putative methyltransferase NSUN4 antibody

RP4-603I14.2 antibody

Shtap antibody

Expand

Images

  • Western blot analysis of NSUN4 on different lysates with Rabbit anti-NSUN4 antibody (<a href="/products/R1706-16" style="font-weight: bold;text-decoration: underline;">R1706-16</a>) at 1/2,000 dilution.<br /><br />Lane 1: MCF7 (Human breast cancer cell) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 42 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1706-16" style="font-weight: bold;text-decoration: underline;">R1706-16</a>, 1/2,000 in 5% NFDM/TBST, 2 hours at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Western blot analysis of NSUN4 on different lysates with Rabbit anti-NSUN4 antibody (R1706-16) at 1/2,000 dilution.

    Lane 1: MCF7 (Human breast cancer cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 42 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1706-16, 1/2,000 in 5% NFDM/TBST, 2 hours at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

  • ICC staining NSUN4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining NSUN4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining NSUN4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining NSUN4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining NSUN4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining NSUN4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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