iFluor™ 594 Conjugated NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07]

Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NeuN (HA720167F).

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded mouse cerebral cortex tissue labeling NeuN (HA720167F).

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling NeuN (HA720167F).

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody NeuN (HA720167F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.