Neuropilin 1 Recombinant Rabbit Monoclonal Antibody [ST05-30] - BSA and Azide free
Catalog# HA750189
Neuropilin 1 Recombinant Rabbit Monoclonal Antibody [ST05-30] - BSA and Azide free
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
IP
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
Neuropilin 1 Recombinant Rabbit Monoclonal Antibody [ST05-30] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Neuropilin-1 aa 881-923 / 923.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Target Molecular Weight
Predicted band size: 103 kDa
Positive Control
U-87 MG cell lysate, A549 cell lysate, MDA-MB-231 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human liver tissue, mouse heart tissue lysate, rat heart tissue lysate, SHG-44, MCF-7, HUVEC, human kidney tissue, mouse kidney tissue.
Conjugation
unconjugated
Clone Number
ST05-30
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Neuropilin is a type I transmembrane receptor that has been implicated in aspects of axon growth and guidance and has been shown to act as a high affinity receptor for class III semaphorins and vascular endothelial growth factor (VEGF). A closely related protein, neuropilin-2, shares a common domain structure and signifcant homology with neuropilin and also acts as a receptor for the class III semaphorins and VEGF. Both neuropilins are involved in regulating many physiological pathways including axonal guidance and angiogenesis, however they exhibit differential expression in the adult vasculature. Neuropilin-2 is polysialylated and expressed on the surface of dendritic cells. It is also expressed by venous and lymphatic endothelium. Neuropilin is expressed predominantly by arterial endothelium.
Background References
1. Liu F et al. Prostate cancer cells induce osteoblastic differentiation via semaphorin 3A. Prostate 75:370-80 (2015).
2. Linthicum FH et al. The periductal channels of the endolymphatic duct, hydrodynamic implications. Otolaryngol Head Neck Surg 150:441-7 (2014).
Sequence Similarity
Belongs to the neuropilin family.
Tissue Specificity
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
Subcellular Location
Cell membrane, Secreted.
Synonyms
A5 protein antibody
BDCA4 antibody
BLOOD DENDRITIC CELL ANTIGEN 4 antibody
CD304 antibody
Neuropilin-1 antibody
Neuropilin1 antibody
NP1 antibody
NPN1 antibody
NRP 1 antibody
NRP antibody
ExpandImages
-
☑ Relative expression (RE)
This data was developed using ET1609-69, the same antibody clone in a different buffer formulation.
Western blot analysis of Neuropilin 1 on different lysates with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: A549 cell lysate
Lane 3: SK-Br-3 cell lysate (low expression)
Lane 4: MDA-MB-231 cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 103 kDa
Observed band size: 130 kDa
Exposure time: 35 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-69) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1609-69, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using ET1609-69, the same antibody clone in a different buffer formulation.
Western blot analysis of Neuropilin 1 on different lysates with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/5,000 dilution.
Lane 1: A549 cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: SK-Br-3 cell lysate (low expression)
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse heart tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat heart tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 103 kDa
Observed band size: 130 kDa
Exposure time: Lane 1-3: 1 minute 21 seconds; Lane 4-7: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-69) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1609-69, the same antibody clone in a different buffer formulation.
ICC staining of Neuropilin 1 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). -
This data was developed using ET1609-69, the same antibody clone in a different buffer formulation.
ICC staining of Neuropilin 1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). -
This data was developed using ET1609-69, the same antibody clone in a different buffer formulation.
ICC staining of Neuropilin 1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). -
This data was developed using ET1609-69, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-69) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1609-69, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Knockdown (KD)
This data was developed using ET1609-69, the same antibody clone in a different buffer formulation.
Western blot analysis of Neuropilin 1 on different lysates with Rabbit anti-Neuropilin 1 antibody (ET1609-69) at 1/5,000 dilution.
Lane 1: MDA-MB-231-si NT cell lysate
Lane 2: MDA-MB-231-si Neuropilin 1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 103 kDa
Observed band size: 130 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-69) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Neuropilin 1 Recombinant Rabbit Monoclonal Antibody [ST05-30]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
