PDGFR beta Recombinant Antibody [SY10-08] - Rat IgG1 (Chimeric)
Catalog# HA601577
PDGFR beta Recombinant Antibody [SY10-08] - Rat IgG1 (Chimeric)
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WB
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IHC-Fr
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PDGFR beta Recombinant Antibody [SY10-08] - Rat IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within Human PDGFR beta aa 961-1,106 / 1,106.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-Fr, IHC-P
Target Molecular Weight
Predicted band size: 124 kDa
Positive Control
SH-SY5Y cell lysate, C2C12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human spleen tissue, mouse spleen tissue, rat spleen tissue.
Conjugation
unconjugated
Clone Number
SY10-08
Reactivity Data
| WB | IHC-Fr | IHC-P | |
|---|---|---|---|
| Human |
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| Mouse |
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|
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:10,000
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IHC-Fr
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1:200
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IHC-P
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1:200-1:1,000
Target
Function
Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and –β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two α receptors, PDGF-AB induces dimerization of αα and αβ and PDGF-BB induces the formation of three types of dimers, αα, αβ and ββ. Translocation of the PDGFR-∫ gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.
Background References
1. Cui Z et al. Endothelial PDGF-BB/PDGFR-beta signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation. Bone Res. 2022 Aug
2. Hu G et al. PDGFR-beta(+) fibroblasts deteriorate survival in human solid tumors: a meta-analysis. Aging (Albany NY). 2021 May
Subcellular Location
Cell membrane, Cytoplasmic vesicle, Lysosome lumen.
Synonyms
Beta platelet derived growth factor receptor antibody
Beta-type platelet-derived growth factor receptor antibody
CD 140B antibody
CD140 antigen-like family member B antibody
CD140b antibody
CD140b antigen antibody
IBGC4 antibody
IMF1 antibody
JTK12 antibody
OTTHUMP00000160528 antibody
ExpandImages
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Western blot analysis of PDGFR beta on different lysates with Rat anti-PDGFR beta antibody (HA601577) at 1/10,000 dilution.
Lane 1: SH-SY5Y cell lysate (10 µg/Lane)
Lane 2: C2C12 cell lysate (10 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Lane 4: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 124 kDa
Observed band size: 190 kDa
Exposure time: 1 minute 34 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601577) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: spleen
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rat anti-PDGFR beta antibody (HA601577) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601577) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rat anti-PDGFR beta antibody (HA601577) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601577) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rat anti-PDGFR beta antibody (HA601577) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601577) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"