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Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750371

Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Paravalbumin aa 1-110 / 110.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr, IF-Tissue, IP

Target Molecular Weight

Predicted band size: 12 kDa

Positive Control

Mouse cerebellum tissue, rat cerebellum tissue, human cerebellum tissue, RPMI 8226 cell lysate, Mouse skeletal muscle tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate.

Conjugation

unconjugated

Clone Number

JM100-08

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Parvalbumin (PV) is a calcium-binding protein with low molecular weight (typically 9-11 kDa). In humans, it is encoded by the PVALB gene. It is not a member of the albumin family; it is named for its size (parv-, from Latin parvus small) and its ability to coagulate. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is found in fast-contracting muscles, where its levels are highest, as well as in the brain and some endocrine tissues. Parvalbumin is a small, stable protein containing EF-hand type calcium binding sites. It is involved in calcium signaling. Calcium binding proteins like parvalbumin play a role in many physiological processes, namely cell-cycle regulation, second messenger production, muscle contraction, organization of microtubules and phototransduction. Therefore, calcium-binding proteins must distinguish calcium in the presence of high concentrations of other metal ions. The mechanism for the calcium selectivity has been extensively studied.

Background References

1. Hijazi S et al. Fast-spiking parvalbumin-positive interneurons in brain physiology and Alzheimer\'s disease. Mol Psychiatry. 2023 Dec

2. Feng YF et al. Parvalbumin neurons mediate neurological phenotypes of anti-NMDAR encephalitis. Brain. 2025 May

Sequence Similarity

Belongs to the parvalbumin family.

Subcellular Location

Axon, cytoplasm, synapse.

UNIPROT

SwissProt: P20472 Human

SwissProt: P32848 Mouse

SwissProt: P02625 Rat

Synonyms

D22S749 antibody

MGC116759 antibody

Parvalbumin alpha antibody

PRVA_HUMAN antibody

PVALB antibody

Images

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Parvalbumin, <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, red); 1/500 (SLC32A1 / VGAT, <a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>, green)<br /><br />Antigen retrieval: Not required

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (Parvalbumin, ET1703-15, red); 1/500 (SLC32A1 / VGAT, HA601384, green)

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (Parvalbumin, <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, red); 1/500 (SLC32A1 / VGAT, <a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>, green)<br /><br />Antigen retrieval: Not required

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (Parvalbumin, ET1703-15, red); 1/500 (SLC32A1 / VGAT, HA601384, green)

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/1,000<br /><br />Antigen retrieval: Not required

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/1,000

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/50,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/50,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Parvalbumin antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Parvalbumin on different lysates with Rabbit anti-Parvalbumin antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/1,000 dilution.<br /><br />Lane 1: RPMI 8226 cell lysate (20 µg/Lane)<br />Lane 2: SK-MEL-28 cell lysate (negative) (20 µg/Lane)<br />Lane 3: Mouse skeletal muscle tissue lysate (20 µg/Lane)<br />Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane)<br />Lane 5: Rat cerebellum tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 12 kDa<br />Observed band size: 14 kDa<br />Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Western blot analysis of Parvalbumin on different lysates with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/1,000 dilution.

    Lane 1: RPMI 8226 cell lysate (20 µg/Lane)
    Lane 2: SK-MEL-28 cell lysate (negative) (20 µg/Lane)
    Lane 3: Mouse skeletal muscle tissue lysate (20 µg/Lane)
    Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane)
    Lane 5: Rat cerebellum tissue lysate (40 µg/Lane)

    Predicted band size: 12 kDa
    Observed band size: 14 kDa
    Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Application: IF-tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • This data was developed using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, the same antibody clone in a different buffer formulation.<br />Parvalbumin was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/025-100-005" style="font-weight: bold;text-decoration: underline;">025-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: RPMI 8226 cell lysate (input)<br />Lane 2: <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a> IP in RPMI 8226 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a> in RPMI 8226 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 10 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    This data was developed using ET1703-15, the same antibody clone in a different buffer formulation.
    Parvalbumin was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with ET1703-15 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1703-15 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: RPMI 8226 cell lysate (input)
    Lane 2: ET1703-15 IP in RPMI 8226 cell lysate
    Lane 3: Rabbit IgG instead of ET1703-15 in RPMI 8226 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 10 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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