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Parvalbumin Recombinant Antibody [JM100-08] - Chicken IgY (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610440

Parvalbumin Recombinant Antibody [JM100-08] - Chicken IgY (Chimeric) - BSA and Azide free

Application

  • WB

  • IHC-Fr

  • IHC-P

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Parvalbumin Recombinant Antibody [JM100-08] - Chicken IgY (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within Human Paravalbumin aa 1-110 / 110.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-Fr, IHC-P, FC(Intra)

Target Molecular Weight

Predicted band size: 12 kDa

Positive Control

RPMI8226 (Human multiple myeloma cell) cell lysate, Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysates.

Conjugation

unconjugated

Clone Number

JM100-08

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:2,000

  • FC(Intra)

  • 1:1,000

Target

Function

Parvalbumin (PV) is a calcium-binding protein with low molecular weight (typically 9-11 kDa). In humans, it is encoded by the PVALB gene. It is not a member of the albumin family; it is named for its size (parv-, from Latin parvus small) and its ability to coagulate. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is found in fast-contracting muscles, where its levels are highest, as well as in the brain and some endocrine tissues. Parvalbumin is a small, stable protein containing EF-hand type calcium binding sites. It is involved in calcium signaling. Calcium binding proteins like parvalbumin play a role in many physiological processes, namely cell-cycle regulation, second messenger production, muscle contraction, organization of microtubules and phototransduction. Therefore, calcium-binding proteins must distinguish calcium in the presence of high concentrations of other metal ions. The mechanism for the calcium selectivity has been extensively studied.

Background References

1. Hijazi S et al. Fast-spiking parvalbumin-positive interneurons in brain physiology and Alzheimer\'s disease. Mol Psychiatry. 2023 Dec

2. Feng YF et al. Parvalbumin neurons mediate neurological phenotypes of anti-NMDAR encephalitis. Brain. 2025 May

Subcellular Location

Axon, cytoplasm, synapse.

UNIPROT

SwissProt: P20472 Human

SwissProt: P32848 Mouse

SwissProt: P02625 Rat

Synonyms

D22S749 antibody

MGC116759 antibody

Parvalbumin alpha antibody

PRVA_HUMAN antibody

PVALB antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Parvalbumin on different lysates with Chicken anti-Parvalbumin antibody (<a href="/products/HA610440" style="font-weight: bold;text-decoration: underline;">HA610440</a>) at 1/5,000 dilution.<br /><br />Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate<br />Lane 2: SK-MEL-28 (Human malignant melanoma cell) cell lysate<br />Lane 3: Mouse skeletal muscle tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1801<br /><br />Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA610440" style="font-weight: bold;text-decoration: underline;">HA610440</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Rabbit anti-Chicken IgY-HRP (<a href="/products/HA1026" style="font-weight: bold;text-decoration: underline;">HA1026</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 12 kDa<br />Observed band size: 12 kDa

    ☑ Relative expression (RE)

    Western blot analysis of Parvalbumin on different lysates with Chicken anti-Parvalbumin antibody (HA610440) at 1/5,000 dilution.

    Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate
    Lane 2: SK-MEL-28 (Human malignant melanoma cell) cell lysate
    Lane 3: Mouse skeletal muscle tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA610440, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Rabbit anti-Chicken IgY-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Western blot analysis of Parvalbumin on rat skeletal muscle tissue lysate with Chicken anti-Parvalbumin antibody (<a href="/products/HA610440" style="font-weight: bold;text-decoration: underline;">HA610440</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA610440" style="font-weight: bold;text-decoration: underline;">HA610440</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Rabbit anti-Chicken IgY-HRP (<a href="/products/HA1026" style="font-weight: bold;text-decoration: underline;">HA1026</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 12 kDa<br />Observed band size: 12 kDa

    Western blot analysis of Parvalbumin on rat skeletal muscle tissue lysate with Chicken anti-Parvalbumin antibody (HA610440) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA610440, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Rabbit anti-Chicken IgY-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Mouse <br />Tissue: Cerebellum <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br /> <br />Primary antibody: <a href="/products/HA610440" style="font-weight: bold;text-decoration: underline;">HA610440</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: HA610440, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Rat <br />Tissue: Cerebellum <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br /> <br />Primary antibody: <a href="/products/HA610440" style="font-weight: bold;text-decoration: underline;">HA610440</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Rat
    Tissue: Cerebellum
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: HA610440, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 1.5 hours at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Cerebellum <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610440" style="font-weight: bold;text-decoration: underline;">HA610440</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1146" style="font-weight: bold;text-decoration: underline;">HA1146</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610440, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1146, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610440" style="font-weight: bold;text-decoration: underline;">HA610440</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1146" style="font-weight: bold;text-decoration: underline;">HA1146</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610440, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1146, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Flow Cytometry (Intra) <br /> <br />Species: Human <br />Sample: SK-MEL-28 (Human malignant melanoma cell)/RPMI8226 (Human multiple myeloma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA610440" style="font-weight: bold;text-decoration: underline;">HA610440</a>(1/1,000, Red), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488), 15 minutes at room temperature. <br /> <br />Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: SK-MEL-28 (Human malignant melanoma cell)/RPMI8226 (Human multiple myeloma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA610440(1/1,000, Red), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 15 minutes at room temperature.

    Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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