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Parvalbumin Recombinant Antibody [JM100-08] - Chicken IgY (Chimeric)

Usd: 385

Specification

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Catalog# HA601645

Parvalbumin Recombinant Antibody [JM100-08] - Chicken IgY (Chimeric)

Application

  • WB

  • IHC-Fr

  • IHC-P

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Parvalbumin Recombinant Antibody [JM100-08] - Chicken IgY (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within Human Paravalbumin aa 1-110 / 110.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-Fr, IHC-P, FC(Intra)

Target Molecular Weight

Predicted band size: 12 kDa

Positive Control

RPMI8226 (Human multiple myeloma cell) cell lysate, Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysates.

Conjugation

unconjugated

Clone Number

JM100-08

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P IHC-Fr FC(Intra) IF-Cell
human
Tested Tested
Not Recommended
 
Tested Tested
Not Recommended
mouse
Tested Tested
Tested Tested
Tested Tested
 
 
rat
Tested Tested
Not Recommended
Tested Tested
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:2,000

  • FC(Intra)

  • 1:1,000

Target

Function

Parvalbumin (PV) is a calcium-binding protein with low molecular weight (typically 9-11 kDa). In humans, it is encoded by the PVALB gene. It is not a member of the albumin family; it is named for its size (parv-, from Latin parvus small) and its ability to coagulate. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is found in fast-contracting muscles, where its levels are highest, as well as in the brain and some endocrine tissues. Parvalbumin is a small, stable protein containing EF-hand type calcium binding sites. It is involved in calcium signaling. Calcium binding proteins like parvalbumin play a role in many physiological processes, namely cell-cycle regulation, second messenger production, muscle contraction, organization of microtubules and phototransduction. Therefore, calcium-binding proteins must distinguish calcium in the presence of high concentrations of other metal ions. The mechanism for the calcium selectivity has been extensively studied.

Background References

1. Hijazi S et al. Fast-spiking parvalbumin-positive interneurons in brain physiology and Alzheimer\'s disease. Mol Psychiatry. 2023 Dec

2. Feng YF et al. Parvalbumin neurons mediate neurological phenotypes of anti-NMDAR encephalitis. Brain. 2025 May

Subcellular Location

Axon, cytoplasm, synapse.

UNIPROT

SwissProt: P20472 Human

SwissProt: P32848 Mouse

SwissProt: P02625 Rat

Synonyms

D22S749 antibody

MGC116759 antibody

Parvalbumin alpha antibody

PRVA_HUMAN antibody

PVALB antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Parvalbumin on different lysates with Chicken anti-Parvalbumin antibody (<a href="/products/HA601645" style="font-weight: bold;text-decoration: underline;">HA601645</a>) at 1/5,000 dilution.<br /><br />Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate<br />Lane 2: SK-MEL-28 (Human malignant melanoma cell) cell lysate<br />Lane 3: Mouse skeletal muscle tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1801<br /><br />Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601645" style="font-weight: bold;text-decoration: underline;">HA601645</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Rabbit anti-Chicken IgY-HRP (<a href="/products/HA1026" style="font-weight: bold;text-decoration: underline;">HA1026</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 12 kDa<br />Observed band size: 12 kDa

    ☑ Relative expression (RE)

    Western blot analysis of Parvalbumin on different lysates with Chicken anti-Parvalbumin antibody (HA601645) at 1/5,000 dilution.

    Lane 1: RPMI8226 (Human multiple myeloma cell) cell lysate
    Lane 2: SK-MEL-28 (Human malignant melanoma cell) cell lysate
    Lane 3: Mouse skeletal muscle tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601645, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Rabbit anti-Chicken IgY-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Western blot analysis of Parvalbumin on rat skeletal muscle tissue lysate with Chicken anti-Parvalbumin antibody (<a href="/products/HA601645" style="font-weight: bold;text-decoration: underline;">HA601645</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601645" style="font-weight: bold;text-decoration: underline;">HA601645</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Rabbit anti-Chicken IgY-HRP (<a href="/products/HA1026" style="font-weight: bold;text-decoration: underline;">HA1026</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 12 kDa<br />Observed band size: 12 kDa

    Western blot analysis of Parvalbumin on rat skeletal muscle tissue lysate with Chicken anti-Parvalbumin antibody (HA601645) at 1/5,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601645, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Rabbit anti-Chicken IgY-HRP (HA1026), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Mouse <br />Tissue: Cerebellum <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br /> <br />Primary antibody: <a href="/products/HA601645" style="font-weight: bold;text-decoration: underline;">HA601645</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: HA601645, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Rat <br />Tissue: Cerebellum <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br /> <br />Primary antibody: <a href="/products/HA601645" style="font-weight: bold;text-decoration: underline;">HA601645</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Rat
    Tissue: Cerebellum
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: HA601645, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 1.5 hours at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Cerebellum <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601645" style="font-weight: bold;text-decoration: underline;">HA601645</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1146" style="font-weight: bold;text-decoration: underline;">HA1146</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601645, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1146, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Kidney <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601645" style="font-weight: bold;text-decoration: underline;">HA601645</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1146" style="font-weight: bold;text-decoration: underline;">HA1146</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601645, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1146, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Flow Cytometry (Intra) <br /> <br />Species: Human <br />Sample: SK-MEL-28 (Human malignant melanoma cell)/RPMI8226 (Human multiple myeloma cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA601645" style="font-weight: bold;text-decoration: underline;">HA601645</a>(1/1,000, Red), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Chicken IgY (iFluor&trade; 488), 15 minutes at room temperature. <br /> <br />Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: SK-MEL-28 (Human malignant melanoma cell)/RPMI8226 (Human multiple myeloma cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA601645(1/1,000, Red), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488), 15 minutes at room temperature.

    Negative expression of Parvalbumin protein in SK-MEL-28 cell is consistent with the predicted expression pattern.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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