Skip to content

Phospho-p38 (T180 + Y182) Rabbit Polyclonal Antibody

Usd:

Bulk Order Quote | Customization Request

Catalog# ER1903-01

Phospho-p38 (T180 + Y182) Rabbit Polyclonal Antibody

Application

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-p38 (T180 + Y182) Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated Synthesised phosphopeptide derived from human p38 MAPK around the phosphorylation site of Thr180/Tyr182: M(p-T)G(p-Y)VA.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P, IF-Tissue

Molecular Weight

42 kDa

Positive Control

Mouse muscle tissue, rat muscle tissue, rat brain tissue, mouse brain tissue, human placenta tissue, HepG2.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at -20℃. Avoid repeated freeze/thaw cycles.

Storage Buffer

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

Target

Function

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases(MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Background References

1. Rosenzweig DH et al. Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis. Osteoarthritis Cartilage 20(12):1591-602 (2012).

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: Q16539 Human

SwissProt: P47811 Mouse

SwissProt: P70618 Rat

Synonyms

CSAID-binding protein antibody

Csaids binding protein antibody

CSBP antibody

CSBP1 antibody

CSBP2 antibody

CSPB1 antibody

Cytokine suppressive anti-inflammatory drug-binding protein antibody

EXIP antibody

MAP kinase 14 antibody

MAP kinase MXI2 antibody

Expand

Images

  • Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-P38 MAPK(Phospho-Thr180/Tyr182) Polyclonal Antibody, Unconjugated (<a href="/products/ER1903-01" style="font-weight: bold;text-decoration: underline;">ER1903-01</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-P38 MAPK(Phospho-Thr180/Tyr182) Polyclonal Antibody, Unconjugated (ER1903-01) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-P38 MAPK(Phospho-Thr180/Tyr182) Polyclonal Antibody, Unconjugated (<a href="/products/ER1903-01" style="font-weight: bold;text-decoration: underline;">ER1903-01</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-P38 MAPK(Phospho-Thr180/Tyr182) Polyclonal Antibody, Unconjugated (ER1903-01) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-P38 MAPK(Phospho-Thr180/Tyr182) Polyclonal Antibody, Unconjugated (<a href="/products/ER1903-01" style="font-weight: bold;text-decoration: underline;">ER1903-01</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-P38 MAPK(Phospho-Thr180/Tyr182) Polyclonal Antibody, Unconjugated (ER1903-01) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P-P38 MAPK) Polyclonal Antibody, Unconjugated (<a href="/products/ER1903-01" style="font-weight: bold;text-decoration: underline;">ER1903-01</a>) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

    Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P-P38 MAPK) Polyclonal Antibody, Unconjugated (ER1903-01) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

metafield image

Phospho-p38 (T180 + Y182) Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Products with the same target and pathway

metafield image

Phospho-p38 (T180) Recombinant Rabbit Monoclonal Antibody [JE77-37]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-p38 (T180) Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-p38 (T180 + Y182) Rabbit Polyclonal Antibody

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated