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Phospho-p38 (T180) Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-51

Phospho-p38 (T180) Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

Predicted reactivity

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-p38 (T180) Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr180 of human P38.

Species Reactivity

Human, Mouse (Predicted: Rat)

Predicted species support after-sales service

Validated Applications

WB

Molecular Weight

Predicted band size: 41 kDa

Positive Control

NIH/3T3 cell lysate, 293 cell lysate treated with UV for 60 minutes, A431 cell lysate treated with UV for 40 minutes, Hela cell lysate treated with anisomycin at 250 ng/ml for 30 minutes, Hela cell lysate treated with UV for 40 minutes.

Conjugation

unconjugated

RRID

AB_3069528

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets.

Background References

1. Deak M. et. al. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 17:4426-4441(1998).

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q16539 Human

Synonyms

CSAID Binding Protein 1 antibody

CSAID binding protein antibody

CSAID-binding protein antibody

Csaids binding protein antibody

CSBP 1 antibody

CSBP 2 antibody

CSBP antibody

CSBP1 antibody

CSBP2 antibody

CSPB1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-p38 (T180) on different lysates with Rabbit anti-Phospho-p38 (T180) antibody (<a href="/products/ER2001-51" style="font-weight: bold;text-decoration: underline;">ER2001-51</a>) at 1/2,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-51" style="font-weight: bold;text-decoration: underline;">ER2001-51</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-p38 (T180) on different lysates with Rabbit anti-Phospho-p38 (T180) antibody (ER2001-51) at 1/2,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-51) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-p38 (T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-51" style="font-weight: bold;text-decoration: underline;">ER2001-51</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: 293 cell lysate, untreated with UV<br />Lane 2: 293 cell lysate, treated with UV for 60 minutes

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-p38 (T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 293 cell lysate, untreated with UV
    Lane 2: 293 cell lysate, treated with UV for 60 minutes

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-p38 (T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-51" style="font-weight: bold;text-decoration: underline;">ER2001-51</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: A431 cell lysate, untreated with UV<br />Lane 2: A431 cell lysate, treated with UV for 40 minutes

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-p38 (T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: A431 cell lysate, untreated with UV
    Lane 2: A431 cell lysate, treated with UV for 40 minutes

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-p38 (T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-51" style="font-weight: bold;text-decoration: underline;">ER2001-51</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Hela cell lysate, untreated<br />Lane 2: Hela cell lysate, treated with anisomycin at 250 ng/ml for 30 minutes <br />Lane 3: Hela cell lysate, treated with UV for 40 minutes

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-p38 (T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate, untreated
    Lane 2: Hela cell lysate, treated with anisomycin at 250 ng/ml for 30 minutes
    Lane 3: Hela cell lysate, treated with UV for 40 minutes

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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