Phospho-p38 (T180 + Y182) Rabbit Polyclonal Antibody
Usd: 315 Special Discount
Specification
Catalog# ER2001-52
Phospho-p38 (T180 + Y182) Rabbit Polyclonal Antibody
-
WB
-
Human
-
Mouse
-
Rat
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unconjugated
Safety datasheet
Overview
Product Name
Phospho-p38 (T180 + Y182) Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr180 and Y182 of human P38.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 41 kDa
Positive Control
NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes cell lysate, HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate, C6 treated with 25μg/mL anisomycin for 30 minutes cell lysate, PC-12 treated with UV for 1 hour cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
Target
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets.
Background References
1. Deak M. et. al. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 17:4426-4441(1998).
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
CSAID Binding Protein 1 antibody
CSAID binding protein antibody
CSAID-binding protein antibody
Csaids binding protein antibody
CSBP 1 antibody
CSBP 2 antibody
CSBP antibody
CSBP1 antibody
CSBP2 antibody
CSPB1 antibody
ExpandCSAID Binding Protein 1 antibody
CSAID binding protein antibody
CSAID-binding protein antibody
Csaids binding protein antibody
CSBP 1 antibody
CSBP 2 antibody
CSBP antibody
CSBP1 antibody
CSBP2 antibody
CSPB1 antibody
Cytokine suppressive anti-inflammatory drug-binding protein antibody
EXIP antibody
MAP kinase 14 antibody
MAP kinase MXI2 antibody
MAP kinase p38 alpha antibody
MAPK 14 antibody
MAPK14 antibody
MAX interacting protein 2 antibody
MAX-interacting protein 2 antibody
Mitogen Activated Protein Kinase 14 antibody
Mitogen activated protein kinase p38 alpha antibody
Mitogen-activated protein kinase 14 antibody
Mitogen-activated protein kinase p38 alpha antibody
MK14_HUMAN antibody
Mxi 2 antibody
MXI2 antibody
p38 ALPHA antibody
p38 antibody
p38 MAP kinase antibody
p38 MAPK antibody
p38 mitogen activated protein kinase antibody
p38ALPHA antibody
p38alpha Exip antibody
PRKM14 antibody
PRKM15 antibody
RK antibody
SAPK2A antibody
Stress-activated protein kinase 2a antibody
CollapseImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-p38 (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 (T180 + Y182) antibody (ER2001-52) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 25μg/mL anisomycin for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 1 minute 29 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-52) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-p38 (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 (T180 + Y182) antibody (ER2001-52) at 1/2,000 dilution.
Lane 1: PC-12 cell lysate (40 µg/Lane)
Lane 2: PC-12 treated with UV for 1 hour cell lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-52) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Kaempferol in a Complex Formula Protects Heart from Jellyfish Venom by Modulating MAPK Signaling
Journal: Toxicon
DOI: 10.1016/j.toxicon.2026.109083
IF: 2.4
Application: WB
Reactivity: Rat
Publish date: 2026 Mar
-
Revisiting the NETs-CYBB-p38 MAPK axis in bronchiectasis: The underlying oxidative injury mechanism
Journal: Biochimica Et Biophysica Acta (Bba) - Molecular Basis Of Disease
DOI: 10.1016/j.bbadis.2026.168236
IF: 4.2
Application: WB
Reactivity: Human
Publish date: 2026 Mar
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Capilliposide from Lysimachia capillipes Hemsl. shows anti-inflammatory and anti-pyretic effects via the MAPK/iNOS/NF-κB pathway
Journal: Journal of Pharmacy and Pharmacology
DOI: 10.1093/jpp/rgag021
IF: 3.2
Application: WB
Reactivity: Mouse
Publish date: 2026 Mar
-
COG133 ameliorates depression-like behaviors in mice by inhibiting p38 MAPK–mediated neuroinflammation
Journal: Brain, Behavior, And Immunity
DOI: 10.1016/j.bbi.2026.106491
IF: 7.6
Application: WB
Reactivity: Mouse
Publish date: 2026 Feb
-
Molecular mechanisms by which Ac-SDKP regulates HSP27 via the p38MAPK/mTOR autophagy pathway to ameliorate pulmonary fibrosis
Journal: CELLULAR SIGNALLING
DOI: 10.1016/j.cellsig.2025.112151
IF: 3.7
Application: IF-tissue
Reactivity: Rat
Publish date: 2025 Sept
-
Synergistic Promotion of Triple-Negative Breast Cancer Tumorigenesis and Metastasis by Oral Polystyrene Nanoplastics Exposure via Alloprevotella-Derived Glutamate and Platelet Activation
Journal: Advanced Science
DOI: 10.1002/advs.202508310
IF: 14.1
Application: WB
Reactivity: Mouse
Publish date: 2025 Sept
-
Discovery and Functional Characterization of a Recombinant Fragment of Human Collagen Type XVII
Journal: Journal Of Agricultural And Food Chemistry
DOI: 10.1021/acs.jafc.5c00277
IF: 5.7
Application: WB
Reactivity: Human
Publish date: 2025 Mar
-
Baicalein antagonises Rhopilema esculentum toxin-induced oxidative stress and apoptosis by modulating ROS-MAPK-NF-κB and inhibiting PLA2 activity
Journal: TOXICON
DOI: 10.1016/j.toxicon.2025.108266
IF: 2.4
Application: WB
Reactivity: Mouse
Publish date: 2025 Jan
-
Baicalein antagonises Rhopilema esculentum toxin-induced oxidative stress and apoptosis by modulating ROS-MAPK-NF-κB and inhibiting PLA2 activity
Journal: Toxicon
DOI:
IF: 2.6
Application: WB
Reactivity: Mouse
Publish date: 2025 Jan
-
Melanoidins From Prunus mume Fruit-Juice Concentrate: Polarity-Driven Differences in Immunomodulatory Activity
Journal: Journal of Food Science
DOI: 10.1111/1750-3841.70749
IF: 3.4
Application: WB
Reactivity: Mouse
Publish date: 2025 Dec
-
Insight of protease and PLA2 activity co-inhibition on jellyfish toxin-induced inflammation and multiple organ dysfunction
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2025.147167
IF: 8.5
Application: WB
Reactivity: Mouse
Publish date: 2025 Aug
-
USP44 Regulates Chemoresistance Induced by ROS and the MAPK/NF-κB Pathway Through the Stabilization of ITGB4 in Gastric Cancer
Journal: FASEB Journal
DOI: 10.1096/fj.202501369R
IF: 4.2
Application: WB
Reactivity: Human
Publish date: 2025 Aug
-
Disrupted betaine metabolism drives Th17 cell differentiation, mediating methamphetamine-induced depressive behaviors in male mice
Journal: Journal Of Neuroinflammation
DOI: 10.1186/s12974-025-03532-1
IF: 10.1
Application: WB
Reactivity: Mouse
Publish date: 2025 Aug
-
Host genetic variation governs PCV2 susceptibility through CXCL13 and ELK1-mediated immune regulation
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2025.143170
IF: 7.7
Application: WB
Reactivity: Pig
Publish date: 2025 Apr
-
Methyl 3,4-dihydrobenzoate attenuates muscle fiber necroptosis and macrophage pyroptosis by regulating oxidative stress in inflammatory myopathies
Journal: International Immunopharmacology
DOI: 10.1016/j.intimp.2025.114608
IF: 4.8
Application: WB
Reactivity: Mouse
Publish date: 2025 Apr
-
GALNT9 enrichment attenuates MPP+-induced cytotoxicity by ameliorating protein aggregations containing α-synuclein and mitochondrial dysfunction
Journal: Biology Direct
DOI: https://doi.org/10.1186/s13062-024-00524-8
IF: 3.8
Application: WB
Reactivity: Rat
Publish date: 2024 Sep
-
The CD163/TWEAK/Fn14 axis: A potential therapeutic target for alleviating inflammatory bone loss
Journal: Journal Of Orthopaedic Translation
DOI:
IF: 5.9
Application: WB
Reactivity: Mouse
Publish date: 2024 Oct
-
MicroRNA-122 protects against interferon-α-induced hepatic inflammatory response via the Janus kinase–signal transducer and activator of transcription pathway
Journal: Endocrine Journal
DOI:
IF: 1.3
Application: WB
Reactivity: Human
Publish date: 2024 Oct
-
Knocking Down PIAS3 Reduces H2O2-induced Oxidative Stress Injury in HT22 Cells
Journal: Cell Biochemistry And Biophysics
DOI:
IF: 2.6
Application: WB
Reactivity: Mouse
Publish date: 2024 May
-
Disrupting PIAS3-mediated SUMOylation of MLK3 ameliorates poststroke neuronal damage and deficits in cognitive and sensorimotor behaviors
Journal: Cellular And Molecular Life Sciences
DOI:
IF: 8
Application: WB
Reactivity: Mouse
Publish date: 2024 Mar
-
Targeting DAD1 gene with CRISPR-Cas9 system transmucosally delivered by fluorinated polylysine nanoparticles for bladder cancer intravesical gene therapy
Journal: Theranostics
DOI: 10.7150/thno.88550
IF: 12.4
Application: WB
Reactivity: Human
Publish date: 2024 Jan
-
Effect and mechanism of recombinant human fibroblast growth factor 18 on osteoporosis in OVX mice
Journal: Climacteric
DOI:
IF: 2.8
Application: WB
Reactivity: Mouse
Publish date: 2024 Feb
-
Low-molecular-weight heparin sodium inhibits the MAPK pathway for psoriasis treatment in mice
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2024.139372
IF: 7.7
Application: WB
Reactivity: Mouse
Publish date: 2024 Dec
-
Bisphenol S exposure interrupted human embryonic stem cell derived cardiomyocytes differentiation through ER-NF-κB/ERK signaling pathway
Journal: Ecotoxicology And Environmental Safety
DOI:
IF: 6.2
Application: WB
Reactivity: Human
Publish date: 2024 Dec
-
Intervention effect of regulating GABA-A receptor activity on the formation of experimental abdominal aortic aneurysm in rats
Journal: Scientific Reports
DOI:
IF: 3.8
Application: WB
Reactivity: Rat
Publish date: 2024 Dec
-
GLP-1RAs attenuated obesity and reversed leptin resistance partly via activating the microbiome-derived inosine/A2A pathway
Journal: Acta Pharmaceutica Sinica B
DOI:
IF: 14.7
Application: WB
Reactivity: Mouse
Publish date: 2024 Dec
-
Comparative Analysis of Tentacle Extract and Nematocyst Venom: Toxicity, Mechanism, and Potential Intervention in the Giant Jellyfish Nemopilema nomurai
Journal: Marine Drugs
DOI:
IF: 4.9
Application: WB
Reactivity: Mouse
Publish date: 2024 Aug
-
Exploring novel indazole derivatives as ASK1 inhibitors: design, synthesis, biological evaluation and docking studies
Journal: Bioorganic Chemistry
DOI:
IF: 5.1
Application: WB
Reactivity: Human
Publish date: 2024 Apr
-
Apigenin Inhibits the Progression of Osteoarthritis by Mediating Macrophage Polarization
Journal: Molecules
DOI:
IF: 4.927
Application: WB
Reactivity: Mouse
Publish date: 2023 Mar
-
SETDB1-mediated CD147-K71 di-methylation promotes cell apoptosis in non-small cell lung cancer
Journal: Genes & Diseases
DOI:
IF: 6.8
Application: WB
Reactivity: Human
Publish date: 2023 Mar
-
EYE-503: A Novel Retinoic Acid Drug for Treating Retinal Neurodegeneration
Journal: Pharmaceuticals
DOI:
IF: 4.6
Application: WB
Reactivity: Mouse
Publish date: 2023 Jul
-
A simple self-assembly nanomicelle based on brain tumor-targeting peptide-mediated siRNA delivery for glioma immunotherapy via intranasal administration
Journal: Acta Biomaterialia
DOI:
IF: 10.633
Application: WB
Reactivity: Mouse
Publish date: 2023 Jan
-
Analyses of Transcriptomics upon IL-1β-Stimulated Mouse Chondrocytes and the Protective Effect of Catalpol through the NOD2/NF-κB/MAPK Signaling Pathway
Journal: Molecules
DOI:
IF: 4.927
Application: WB
Reactivity: Mouse
Publish date: 2023 Feb
-
Limonin delays the progression of intervertebral disc degeneration in vivo and in vitro: the key role of the MAPK/NF-κB and necroptosis pathways
Journal: Journal Of Pharmacy And Pharmacology
DOI:
IF: 3.3
Application: WB
Reactivity: Mouse
Publish date: 2023 Apr
-
Downregulation of AC092894. 1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis
Journal: BMC Medicine
DOI:
IF: 9.3
Application: WB
Reactivity: Human
Publish date: 2023 Apr
-
Selumetinib - a potential small molecule inhibitor for osteoarthritis treatment
Journal: Frontiers In Pharmacology
DOI:
IF: 5.988
Application: WB
Reactivity: Mouse
Publish date: 2022 Sept
-
Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota
Journal: Gut Microbes
DOI:
IF: 10.245
Application: WB
Reactivity: Pig
Publish date: 2022 May
-
Melatonin improves bisphenol A-induced cell apoptosis, oxidative stress and autophagy impairment via inhibition of the p38 MAPK signaling pathway in FLK-BLV cells
Journal: Environmental Toxicology
DOI:
IF: 4.109
Application: WB
Reactivity: Cow
Publish date: 2022 Jun
-
Single-Cell Sequencing Yields Insights in the Evolution of Foot-and-Mouth Disease Virus Persistent Infection
Journal: Frontiers In Cellular And Infection Microbiology
DOI:
IF: 6.073
Application: WB
Reactivity: Hamster
Publish date: 2022 Jul
-
CD147 Mediates 5-Fluorouracil Resistance in Colorectal Cancer by Reprogramming Glycolipid Metabolism
Journal: Frontiers In Oncology
DOI:
IF: 5.738
Application: WB
Reactivity: Human
Publish date: 2022 Jul
-
Platelet-Derived Growth Factor-BB Inhibits Intervertebral Disc Degeneration via Suppressing Pyroptosis and Activating the MAPK Signaling Pathway
Journal: Frontiers In Pharmacology
DOI:
IF: 4.220
Application: WB
Reactivity: Human
Publish date: 2022 Jan
-
AZ-628 delays osteoarthritis progression via inhibiting the TNF-α-induced chondrocyte necroptosis and regulating osteoclast formation
Journal: International Immunopharmacology
DOI:
IF: 5.714
Application: WB
Reactivity: Mouse
Publish date: 2022 Aug
-
Hyperglycemia modulates M1/M2 macrophage polarization via reactive oxygen species overproduction in ligature-induced periodontitis
Journal: Journal Of Periodontal Research
DOI:
IF: 4.42
Application: WB
Reactivity: Mouse
Publish date: 2021 Oct
-
Riboflavin as a Mucosal Adjuvant for Nasal Influenza Vaccine
Journal: Vaccine
DOI:
IF: 4.090
Application: WB
Reactivity: Mouse
Publish date: 2021 Nov
-
Purification of recombinant human fibroblast growth factor 13 in E. coli and its molecular mechanism of mitogenesis
Journal: Applied Microbiology And Biotechnology
DOI:
IF: 3.67
Application: WB
Reactivity: Mouse
Publish date: 2019 Sep
Products with the same target and pathway
Phospho-p38 (T180) Recombinant Rabbit Monoclonal Antibody [JE77-37]
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Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Phospho-p38 (T180) Rabbit Polyclonal Antibody
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Conjugate: unconjugated
Phospho-p38 (T180 + Y182) Rabbit Polyclonal Antibody
Application: IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated