ABCA1 Rabbit Polyclonal Antibody (R1510-42)
Catalog# R1510-42
Rabbit Polyclonal to ABCA1
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: Rat brain tissue lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Predicted band size: 254 kDa
Observed band size: 254 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-42) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
ABCA1 Rabbit Polyclonal Antibody (R1510-42)
Immunogen
Synthetic peptide within human abca1 aa 21-70 / 2,261.
Host
Rabbit
Positive Control
Rat brain tissue lysate, Jurkat cell lysate, mouse brain tissue lysate, PC-3M, human liver tissue, human pancreas tissue, mouse prostate tissue, mouse pancreas tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 254 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ABCA1
SYNONYMS
ABC 1 antibody;ABC Transporter 1 antibody;ABC-1 antibody;ABC1 antibody;ABCA 1 antibody;ABCA1 antibody;ABCA1_HUMAN antibody;ATP binding Cassette 1 antibody;ATP binding cassette sub family A ABC1 member 1 antibody;ATP binding cassette sub family A member 1 antibody;ATP binding Cassette Transporter 1 antibody;ATP-binding cassette 1 antibody;ATP-binding cassette sub-family A member 1 antibody;ATP-binding cassette transporter 1 antibody;CERP antibody;Cholesterol efflux regulatory protein antibody;FLJ14958 antibody;HDLDT1 antibody;Membrane bound antibody;MGC164864 antibody;MGC165011 antibody;TD antibody;TGD antibody
SEQUENCE SIMILARITIES
Belongs to the ABC transporter superfamily. ABCA family.
TISSUE SPECIFICITY
Widely expressed, but most abundant in macrophages.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation on Ser-2054 regulates phospholipid efflux.; Palmitoylation by DHHC8 is essential for membrane localization.
SUBCELLULAR LOCATION
Membrane
FUNCTION
ABC1 (for ATP-binding cassette transporter 1) is a member of the family of ATP-binding cassette proteins which transport various molecules across biological membranes. ABC1 contains two characteristic ATP-binding domains and 12 transmembrane domains which form a channel-like structure for transport. Mutations in the ABC1 gene are implicated in Tangier disease, characterized by low serum high density lipoprotein. ABC1 is widely expressed in human tissues, with high levels of expression in liver, lung, adrenal glands, placenta and fetal tissue. ABC1 expression is induced during monocyte differentiation and upregulated in the presence of acetylated low-density lipoprotein. ABC1 may have a dual regulatory function in macrophage lipid metabolism and inflammation.
CITATIONS
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Wang, M. S., Han, Q. S., Jia, Z. R., Chen, C. S., Qiao, C., Liu, Q. Q., Zhang, Y. M., Wang, K. W., Wang, J., Xiao, K., & Ding, X. S.
PPARα agonist fenofibrate relieves acquired resistance to gefitinib in non-small cell lung cancer by promoting apoptosis via PPARα/AMPK/AKT/FoxO1 pathway. Acta pharmacologica Sinica, 10.1038/s41401-021-00638-z. Advance online publication.
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WB
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Human
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Citation
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