AFP Rabbit Polyclonal Antibody
Catalog# R1601-1
AFP Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Rat
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Mouse
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unconjugated
Overview
Product Name
AFP Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within N-terminal human AFP.
Species Reactivity
Human, Rat (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 69 kDa
Positive Control
MCF-7 cell lysate, PC-12 cell lysate, human liver tissue lysate, HepG2, MCF-7, Hela, human liver cancer tissue, human breast cancer tissue, 293T.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
Target
Function
α-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity.
Background References
暂无
Sequence Similarity
Belongs to the ALB/AFP/VDB family.
Tissue Specificity
Plasma. Synthesized by the fetal liver and yolk sac.
Post-translational Modification
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.; Sulfated.
Subcellular Location
Secreted.
Synonyms
Afp antibody
AFPD antibody
Alpha fetoglobulin antibody
Alpha fetoprotein antibody
Alpha fetoprotein precursor antibody
Alpha-1-fetoprotein antibody
Alpha-fetoglobulin antibody
Alpha-fetoprotein antibody
alpha-fetoprotein, Hereditary persistence of, included antibody
FETA antibody
ExpandImages
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Western blot analysis of AFP on Hep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-AFP antibody (R1601-1) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: R1601-1, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 69 kDa
Observed band size: 69 kDa -
ICC staining AFP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining AFP in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining AFP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
AFP Recombinant Rabbit Monoclonal Antibody [PSH11-75]
Application: WB,IF-Cell,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
AFP Recombinant Rabbit Monoclonal Antibody [PD00-71]
Application: IHC-P,WB
Reactivity: Human
Conjugate: unconjugated
AFP Mouse Monoclonal Antibody [A5-G3]
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
AFP Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human
Conjugate: unconjugated