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HDAC2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1601-7

HDAC2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

HDAC2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within C-terminal human Histone Deacetylase 2.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 55 kDa

Positive Control

SH-SY5Y cell lysate, 293T cell lysate, Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, COS-7 cell lysate, Wild-type Hela whole cell lysate, LOVO, NIH/3T3, SH-SY5Y, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse brain tissue.

Conjugation

unconjugated

RRID

AB_3073405

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:10,000

  • IF-Cell

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:50-1:100

Target

Function

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Background References

暂无

Sequence Similarity

Belongs to the histone deacetylase family. HD type 1 subfamily.

Tissue Specificity

Widely expressed; lower levels in brain and lung.

Post-translational Modification

S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q92769 Human

SwissProt: P70288 Mouse

SwissProt: B1WBY8 Rat

Synonyms

D10Wsu179e antibody

HD 2 antibody

HD2 antibody

HDAC 2 antibody

Hdac2 antibody

HDAC2_HUMAN antibody

Histone deacetylase 2 (HD2) antibody

Histone deacetylase 2 antibody

OTTHUMP00000017046 antibody

OTTHUMP00000227077 antibody

Expand

Images

  • Western blot analysis of HDAC2 on different lysates with Rabbit anti-HDAC2 antibody (<a href="/products/R1601-7" style="font-weight: bold;text-decoration: underline;">R1601-7</a>) at 1/10,000 dilution.<br /><br />Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate<br />Lane 2: 293T (Human embryonic kidney cell) cell lysate<br />Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate<br />Lane 5: NIH/3T3 (Mouse fibroblast) cell lysate<br />Lane 6: COS-7 (African green monkey kidney fibroblast) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 10 seconds ; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/R1601-7" style="font-weight: bold;text-decoration: underline;">R1601-7</a>, 1/10,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 55 kDa<br />Observed band size: 55 kDa

    Western blot analysis of HDAC2 on different lysates with Rabbit anti-HDAC2 antibody (R1601-7) at 1/10,000 dilution.

    Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
    Lane 2: 293T (Human embryonic kidney cell) cell lysate
    Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
    Lane 5: NIH/3T3 (Mouse fibroblast) cell lysate
    Lane 6: COS-7 (African green monkey kidney fibroblast) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds ; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: R1601-7, 1/10,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of HDAC2 with anti-HDAC2 antibody (<a href="/products/R1601-7" style="font-weight: bold;text-decoration: underline;">R1601-7</a>) at 1/500 dilution.<br />Lane 1: Wild-type Hela whole cell lysate (10 µg).<br />Lane 2/3: HDAC2 knockout Hela whole cell lysate (10 µg).<br /><br /><a href="/products/R1601-7" style="font-weight: bold;text-decoration: underline;">R1601-7</a> was shown to specifically react with HDAC2 in wild-type Hela cells. No bands were observed when HDAC2 knockout sample were tested. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1601-7" style="font-weight: bold;text-decoration: underline;">R1601-7</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    All lanes: Western blot analysis of HDAC2 with anti-HDAC2 antibody (R1601-7) at 1/500 dilution.
    Lane 1: Wild-type Hela whole cell lysate (10 µg).
    Lane 2/3: HDAC2 knockout Hela whole cell lysate (10 µg).

    R1601-7 was shown to specifically react with HDAC2 in wild-type Hela cells. No bands were observed when HDAC2 knockout sample were tested. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1601-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • ICC staining HDAC2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining HDAC2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining HDAC2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining HDAC2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining HDAC2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining HDAC2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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