TIMP4 Recombinant Rabbit Monoclonal Antibody [PSH20-91]
Catalog# HA724186
TIMP4 Recombinant Rabbit Monoclonal Antibody [PSH20-91]
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WB
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FC
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Human
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Mouse
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Rat
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Green monkey
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HA751773
不含抗保成分
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unconjugated
Overview
Product Name
TIMP4 Recombinant Rabbit Monoclonal Antibody [PSH20-91]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TIMP4 aa 1-224.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 26 kDa
Positive Control
HeLa cell lysate, U-87 MG cell lysate, HT-29 cell lysate, MDA-MB-231 cell lysate, HEK-293 cell lysate, BxPC-3 cell lysate, Raji cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, U-87 MG.
Conjugation
unconjugated
Clone Number
PSH20-91
Reactivity Data
| WB | FC | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
| Green Monkey |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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FC
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1:1,000
Target
Function
Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.
Background References
1. Zhou L et al. TIMP4 serves as a novel potential prognostic biomarker for oral squamous cell carcinoma. Sci Rep. 2025 Feb
2. Zhou W et al. Defect of TIMP4 Is Associated with High Myopia and Participates in Rat Ocular Development in a Dose-Dependent Manner. Int J Mol Sci. 2023 Nov
Subcellular Location
Secreted.
Synonyms
Metalloproteinase inhibitor 4 antibody
TIMP 4 antibody
TIMP metallopeptidase inhibitor 4 antibody
TIMP-4 antibody
Timp4 antibody
TIMP4_HUMAN antibody
Tissue inhibitor of metalloproteinase 4 antibody
Tissue inhibitor of metalloproteinases 4 antibody
Images
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Western blot analysis of TIMP4 on different lysates with Rabbit anti-TIMP4 antibody (HA724186) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 5: HEK-293 cell lysate (20 µg/Lane)
Lane 6: BxPC-3 cell lysate (20 µg/Lane)
Lane 7: Raji cell lysate (20 µg/Lane)
Lane 8: COS-1 cell lysate (20 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724186) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of TIMP4 on different lysates with Rabbit anti-TIMP4 antibody (HA724186) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Mouse heart tissue lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: Rat heart tissue lysate (20 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724186) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of U-87 MG cells labeling TIMP4.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA724186, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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TIMP4 Recombinant Rabbit Monoclonal Antibody [PSH20-91] - BSA and Azide free
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Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
