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TIMP4 Recombinant Rabbit Monoclonal Antibody [PSH20-91] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751773

TIMP4 Recombinant Rabbit Monoclonal Antibody [PSH20-91] - BSA and Azide free

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

TIMP4 Recombinant Rabbit Monoclonal Antibody [PSH20-91] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human TIMP4 aa 1-224.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 26 kDa

Positive Control

HeLa cell lysate, U-87 MG cell lysate, HT-29 cell lysate, MDA-MB-231 cell lysate, HEK-293 cell lysate, BxPC-3 cell lysate, Raji cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, U-87 MG.

Conjugation

unconjugated

Clone Number

PSH20-91

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • FC

  • 1:1,000

Target

Function

Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Background References

1. Zhou L et al. TIMP4 serves as a novel potential prognostic biomarker for oral squamous cell carcinoma. Sci Rep. 2025 Feb

2. Zhou W et al. Defect of TIMP4 Is Associated with High Myopia and Participates in Rat Ocular Development in a Dose-Dependent Manner. Int J Mol Sci. 2023 Nov

Subcellular Location

Secreted.

UNIPROT

SwissProt: Q99727 Human

SwissProt: Q9JHB3 Mouse

SwissProt: P81556 Rat

Synonyms

Metalloproteinase inhibitor 4 antibody

TIMP 4 antibody

TIMP metallopeptidase inhibitor 4 antibody

TIMP-4 antibody

Timp4 antibody

TIMP4_HUMAN antibody

Tissue inhibitor of metalloproteinase 4 antibody

Tissue inhibitor of metalloproteinases 4 antibody

Images

  • Western blot analysis of TIMP4 on different lysates with Rabbit anti-TIMP4 antibody (<a href="/products/HA751773" style="font-weight: bold;text-decoration: underline;">HA751773</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: U-87 MG cell lysate (20 µg/Lane)<br />Lane 3: HT-29 cell lysate (20 µg/Lane)<br />Lane 4: MDA-MB-231 cell lysate (20 µg/Lane)<br />Lane 5: HEK-293 cell lysate (20 µg/Lane)<br />Lane 6: BxPC-3 cell lysate (20 µg/Lane)<br />Lane 7: Raji cell lysate (20 µg/Lane)<br />Lane 8: COS-1 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751773" style="font-weight: bold;text-decoration: underline;">HA751773</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TIMP4 on different lysates with Rabbit anti-TIMP4 antibody (HA751773) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: U-87 MG cell lysate (20 µg/Lane)
    Lane 3: HT-29 cell lysate (20 µg/Lane)
    Lane 4: MDA-MB-231 cell lysate (20 µg/Lane)
    Lane 5: HEK-293 cell lysate (20 µg/Lane)
    Lane 6: BxPC-3 cell lysate (20 µg/Lane)
    Lane 7: Raji cell lysate (20 µg/Lane)
    Lane 8: COS-1 cell lysate (20 µg/Lane)

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 4 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751773) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of TIMP4 on different lysates with Rabbit anti-TIMP4 antibody (<a href="/products/HA751773" style="font-weight: bold;text-decoration: underline;">HA751773</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 2: Mouse heart tissue lysate (20 µg/Lane)<br />Lane 3: Rat brain tissue lysate (20 µg/Lane)<br />Lane 4: Rat heart tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 26 kDa<br />Observed band size: 26 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751773" style="font-weight: bold;text-decoration: underline;">HA751773</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TIMP4 on different lysates with Rabbit anti-TIMP4 antibody (HA751773) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate (20 µg/Lane)
    Lane 2: Mouse heart tissue lysate (20 µg/Lane)
    Lane 3: Rat brain tissue lysate (20 µg/Lane)
    Lane 4: Rat heart tissue lysate (20 µg/Lane)

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

    Exposure time: 4 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751773) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of U-87 MG cells labeling TIMP4.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA751773" style="font-weight: bold;text-decoration: underline;">HA751773</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of U-87 MG cells labeling TIMP4.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA751773, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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