Wee1 Recombinant Rabbit Monoclonal Antibody [PSH05-11] - BSA and Azide free
Catalog# HA750984
Wee1 Recombinant Rabbit Monoclonal Antibody [PSH05-11] - BSA and Azide free
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WB
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IHC-P
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Human
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Green monkey
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HA722227
含抗保成分
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unconjugated
Overview
Product Name
Wee1 Recombinant Rabbit Monoclonal Antibody [PSH05-11] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Wee1 aa 251-600 / 646.
Species Reactivity
Human, Green monkey
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 72 kDa
Positive Control
HeLa cell lysate, Raji cell lysate, 293T cell lysate, HT-29 cell lysate, Jurkat cell lysate, COS-1 cell lysate, human testis tissue.
Conjugation
unconjugated
Clone Number
PSH05-11
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:200
Target
Function
Wee1 is a nuclear kinase belonging to the Ser/Thr family of protein kinases in the fission yeast Schizosaccharomyces pombe (S. pombe). Wee1 has a molecular mass of 96 kDa and is a key regulator of cell cycle progression. It influences cell size by inhibiting the entry into mitosis, through inhibiting Cdk1. Wee1 has homologues in many other organisms, including mammals. The regulation of cell size is critical to ensure functionality of a cell. Besides environmental factors such as nutrients, growth factors and functional load, cell size is also controlled by a cellular cell size checkpoint. Wee1 is a component of this checkpoint. It is a kinase determining the timepoint of entry into mitosis, thus influencing the size of the daughter cells. Loss of Wee1 function will produce smaller than normal daughter cell, because cell division occurs prematurely.
Background References
1. Kong A et al. WEE1 Inhibitor: Clinical Development. Curr Oncol Rep. 2021 Jul
2. Taniguchi H et al. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022 May
Subcellular Location
Nucleus.
UNIPROT
Synonyms
DKFZp686I18166 antibody
EC 2.7.10.2 antibody
FLJ16446 antibody
MGC105683 antibody
OTTHUMP00000231338 antibody
OTTHUMP00000231339 antibody
Wee 1 antibody
WEE 1 homolog 1 (S. pombe) antibody
WEE1 antibody
WEE1 homolog (S. pombe) antibody
ExpandImages
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Western blot analysis of Wee1 on different lysates with Rabbit anti-Wee1 antibody (HA750984) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Raji cell lysate
Lane 3: 293T cell lysate
Lane 4: HT-29 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 90 kDa
Exposure time: 43 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750984) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Wee1 antibody (HA750984) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750984) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"